口腔疾病防治2018，Vol.26Issue(3)：166-170,5.DOI:10.12016/j.issn.2096-1456.2018.03.005
改良组织块酶消化法原代培养人牙髓干细胞的研究
Primary culture of hPDSCs with modified enzymatic digestion-explants method
摘要
Abstract
Objective To explore methods using modified tissue enzymatic separations for culturing primary hDP-SCs in vitro and further identify the cells produced. Methods Primary hDPSCs were cultured using the modified tis-sue enzymatic separation method, and cells were identified by morphology, cell surface markers, and differentiation po-tential and evaluated using flow cytometry and growth curves. Results The hDPSCs were successfully isolated using the modified tissue enzymatic separation method. The morphology of these cells was similar to that of fibroblasts and mesenchymal stem cells, and the growth curve was "S" -type. The results of cell phenotype analysis indicated that the cells were positive for surface markers of mesenchymal stem cells, including CD29, CD44, and CD90, and negative for markers of hematopoietic stem cells, including CD34, CD45, and CD106. The cells were capable of differentiating into multiple cell types. Conclusion The modified tissue enzymatic separation method can successful be used to culture primary hDPSCs in vitro.关键词
人牙髓干细胞/组织块酶消化法/原代培养/细胞鉴定Key words
hDPSCs/Explants method and enzymatic separation method/Primary culture/Cells identification分类
医药卫生引用本文复制引用
刘影,高杰,吴补领..改良组织块酶消化法原代培养人牙髓干细胞的研究[J].口腔疾病防治,2018,26(3):166-170,5.基金项目
广东省自然科学基金项目(2015A030310071) （2015A030310071）
广东省医学科学技术研究基金项目(A2016212) （A2016212）
广东省自筹经费类科技计划项目(粤科规财字[2015]110号) （粤科规财字[2015]110号）
