南方农业学报2018，Vol.49Issue(2)：375-382,8.DOI:10.3969/j.issn.2095-1191.2018.02.26
无乳链球菌感染吉富品系尼罗罗非鱼的病理学研究
Pathological research of GIFT tilapia infected by Streptococcus agalactiae
摘要
Abstract
[Objective]The infection approach of Streptococcus agalactiae to GIFT tilapia and target organs were stu-died to provide reference for disease resistant breeding and development of S.agalactiaevaccine.[Method]The GIFT tila-pia was inoculated by S.agalactiae through intraperitoneal injection,gavage and soak,and samples of gill,spleen,liver and small gut were collected for pathomorphology observation.Immunohistochemical location was conducted using anti-S.agalactiae serum of rabbit to research the distribution regulations in each tissues under various inoculation approaches and the inoculated target organs.[Result]The three artificial inoculation methods could all lead to S.agalactiae inocula-tion of GIFT tilapia.In intraperitoneal injection and gavage inoculations,pathological changes occured after 2 h of inocu-lation,but in soak inoculation,pathological changes occured after 5 h,and the degree of pathological changes was less serious than the other two.Immunohistochemical localization results showed that,in intraperitoneal injection treatment, the order of pathogenic bacteria occured in GIFT tilapia was spleen→liver and gill→small gut;the order in gavage inocu-lation was small gut→gill and spleen→liver;and the order in soak inoculation was gill→spleen→liver and small gut.[Conclusion]Intraperitoneal injection,gavage and soak can all lead to S.agalactiae inoculation in GIFT tilapia.The sig-nal of pathogenic bacteria would appear first in spleen,gut and gill.Therefore,the effective measures to control the out-break of S.agalactiaedisease in GIFT tilapia under natural aquaculture conditions are preventing the aquaculture water and food from infection of S.agalactiae.关键词
吉富品系尼罗罗非鱼/无乳链球菌/致病途径/靶器官/免疫组织化学Key words
GIFT tilapia/Streptococcus agalactiae/pathogenic pathway/target organ/immunohistochemistry分类
农业科技引用本文复制引用
罗伟,甘西,朱佳杰,敖秋桅,谭芸,陈明,罗永巨..无乳链球菌感染吉富品系尼罗罗非鱼的病理学研究[J].南方农业学报,2018,49(2):375-382,8.基金项目
广西科技重大专项项目(桂科AA17204080-2) （桂科AA17204080-2）
广西重点研发计划项目(桂科AB16380077) （桂科AB16380077）
国家特色淡水鱼产业技术体系建设项目(CARS-46) （CARS-46）
广西八桂学者专项项目(桂科政字〔2013〕25号) （桂科政字〔2013〕25号）
