环境工程学报2018，Vol.12Issue(2)：544-551,8.DOI:10.12030/j.cjee.201706224
一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响
Screening of one strain of denitrifying phosphorus accumulation bacteria (DPAB) and inhibitions effects of nitrogen-phosphorus removal
摘要
Abstract
In order to attribute to effective and low energy-consumption sewage treatment technology,we screened one strain of DPAB and determined the best inoculation ratio experiments.Using anaerobic sludge as the inoculated sludge,one strain,N-12,was obtained through a series of experiments,including phosphate uptake,nitrate reduction to gas,auxiliary dye granular straining and PHB.Besides,the strain was identified as Acinetobacter by 16S rDNA gene analysis.Then,draw the growth curve and set six different initial inoculation ratios as the measurement of microbial biomass,0％,1％,2％,5％,10％ and 15％,respectively,aim to study the inhibitions effects of nitrogen-phosphorus removal.The results showed that the most efficient was the 2％ system.Results of the correlation analysis,among the removal rates of phosphate and nitrate,OD600 and pH,showed that there was a significant positive correlation coefficient between phosphate removal rate and nitrate removal rate (Person correlation coefficient =0.826,P ＜ 0.01),and they both had significant positive correlation with OD600 and pH.关键词
反硝化聚磷菌(DPAB)/接种比/OD600/脱氮除磷/相关性分析Key words
denitrifying phosphorus accumulation bacteria (DPAB)/inoculation ratios/OD600/nitrogen and phosphorus removal/correlation analysis分类
资源环境引用本文复制引用
吴晓娜,王助贫,谢恩,任梦丽,刘婷婷,郑蕾,丁爱中..一株反硝化聚磷菌筛选及其接种量对脱氮除磷效应的影响[J].环境工程学报,2018,12(2):544-551,8.基金项目
北京市科技计划项目(D161100000216001) （D161100000216001）
国家自然科学基金资助项目(51509003) （51509003）
