环境工程学报2018，Vol.12Issue(2)：670-676,7.DOI:10.12030/j.cjee.201706234
膜生物反应器中小球藻生物膜生长与代谢的光调控特性
Regulation of light intensity on characteristics of growth and metabolism of Chlorella biofilm in a membrane bioreactor
摘要
Abstract
In this study,a gas-liquid separation biofilm cultivation system was developed for improving the transportation performance of light and gas during immobilization culture of Chlorella sp.Biofilm formation of Chlorella sp.and its metabolic performance were investigated under different illumination intensity.Biomass productivity and biochemical composition,as well as chlorophyll,and fatty acid profile were measured to evaluate the characteristics of formation,growth,and lipid synthesis of biofilm under different illumination conditions.High light stress positively regulated cellular lipids accumulation,and the maximal biomass productivity (5.50 g · (m2 · d)-1),lipid productivity (1.71 g· (m2 · d)-1) were obtained at 230 μmol· (m2 · s)-1 illumination intensity.The contents of chlorophyll a and b,as well as starch,increased initially and then decreased,while the contents of carotenoids and proteins became relatively constant.Furthermore,the maximal contents of C16 to C18 of the total fatty acids and unsaturated fatty acids were obtained at 46 μmol · (m2 · s)-1 illumination intensity,while the contents of other lipids rose gradually with an increase of the illumination intensity.关键词
小球藻/气液分离/光照强度/细胞组分Key words
Chlorella/gas-liquid separation/illumination intensity/cell composition分类
资源环境引用本文复制引用
李春,王胜威,张磊,王永忠..膜生物反应器中小球藻生物膜生长与代谢的光调控特性[J].环境工程学报,2018,12(2):670-676,7.基金项目
国家自然科学基金面上项目(51376200) （51376200）
