中国实用神经疾病杂志2018，Vol.21Issue(1)：7-11,5.DOI:10.12083/SYSJ.2018.01.002
p38MAPK在脑缺血后处理大鼠海马区的表达与意义
Expression and significance of p38MAPK in hippocampus following cerebral ischemic postconditioning in rats
摘要
Abstract
Objective To observe the expression change of p38MAPK in hippocampus neurons and investigate the brain protecting mechanism following cerebral ischemic postconditioning in rats .Methods 96 male SD rats were randomly divided into three groups :sham group ,global cerebral ischemia group and cerebral ischemic postconditioning group .The rats in sham group re-ceived head skin cut ,no electric coagulation and carotid separation with no embed wire clip .The model of cerebral ischemia was es-tablished by using improved pulsinelli four vessels block (4-VO) ,and the time of ischemia was 20 minutes .The rats in ischemic postconditioning group were treated with 3 times reperfusion(15s)/ischemia(15s) before reperfusion .Each group was divide into four subgroups (each with 8 rats) according to reperfusion after 6h ,24h ,48h and 72h .We used HE staining under light microscope to observe variation of neurons form and immunohistochemistry and Western blot to test the expression of p 38MAPK .Results The neurons structure of the cerebral ischemia group was damaged ,neuron necrosis rate and the expression of phosphorylated p38MAPK increased at each time point ,the differences were statistically significant (P<0.05) .Compared with cerebral ischemia group ,the neurons structure in the cerebral ischemic postconditioning group were ameliorate damaged ,neuron necrosis rate and the expression of phosphorylated p38MAPK were decreased at each time point ,the difference was statistically significant(P<0.05) . Conclusion Cerebral ischemic postconditioning have endogenous nerve protective effect in cerebral ischemia reperfusion injury ,the mechanism may be related to the decrease of the expression of p38MAPK .关键词
p38MAPK/脑缺血再灌注损伤/脑缺血后处理/海马区/大鼠/脑保护Key words
p38MAPK/Cerebral ischemia/reperfusion injury/Ischemic postconditioning/Hippocampus/Rats/Brain protec-tion分类
医药卫生引用本文复制引用
孙鸿雁,刘宇..p38MAPK在脑缺血后处理大鼠海马区的表达与意义[J].中国实用神经疾病杂志,2018,21(1):7-11,5.基金项目
天津市卫生计生委中西医合基金 ,编号 :13085 （）
