中国实用神经疾病杂志2018，Vol.21Issue(2)：117-123,7.DOI:10.12083/SYSJ.2018.02.031
腹腔注射脂多糖对幼鼠癫痫发作及海马TLR4/HMGB1/P-IκB-α表达的影响
The influnces of Intraperitoneal injection of LPS on infant rats epileptic seizures and expression of TLR 4/HMGB1/P-IκB-α in Hippocampus
摘要
Abstract
Objective To investigate the effect of pretreatment with lipopolysaccharide (LPS) on infant rats epileptic sei-zures and the expression of inflammatory mediators such as TLR4/HMGB1/P-IκB-αin hippocampus .Methods The rats at post-natal 21th day (21d) were randomly divided into saline control group (control group) ,model Ⅰ and model Ⅱ group .The model groups were induced into epilepsy model by kainic acid (KA) ,and the model Ⅱ group were pretreated with intraperitoneal injection of LPS 2 hours before KA application .Behavior of infant rats with epilepsy seizures were observed and given scores according to Racine score ,and the gene expression of TLR4 and HMGB1 at 3h and 24h after SE were detected by fluorescence quantitative PCR ,and the protein expression of TLR4 ,HMGB1 ,P-IκB-αat 3h and 24h after SE were detected by western blot .Results Com-pared to control group ,the TLR4 gene of model group Ⅰ and model group Ⅱ at 3th hour after SE in hippocampus were signifi-cantly increased (t=4.806 ,P<0.05 ;t=4.954 ,P<0.05) ,and also increased significantly at 24th hour after SE (t=3.924 ,P<0.05 ;t=3.792 ,P<0.05) ,and the TLR4 protein expression level of model group Ⅰ and model group Ⅱ at 3th hour after SE in hippocampus were significantly increased(t=7.804 ,P<0.05 ;t=8.385 ,P<0.05) ,and also increased significantly at 24th hour after SE (t=4.256 ,P<0.05 ;t=4.262 ,P<0.05) .At the same time ,the HMGB1 gene of model group Ⅰ and model group Ⅱ at 3th hour after SE in hippocampus were significantly increased (t=3.626 ,P<0.05 ;t=5.255 ,P<0.05) ,and also increased signifi-cantly at 24th hour after SE (t=4.046 ,P<0.05 ;t=2.836 ,P<0.05) ,but the HMGB1 protein expression level of model groupⅠ and model group Ⅱ were neither significantly increased at 3th hour after SE(t=0.389 ,P>0.05 ;t=0.213 ,P>0.05) nor sig-nificantly increased at 24th hour after SE(t=0.106 ,P>0.05 ;t=0.279 ,P>0.05) .What's more ,the P-IκB-α protein expression level of model group Ⅰ and model group Ⅱ at 3th hour after SE in hippocampus were significantly increased (t=4.383 ,P<0.05 ;t=6.627 ,P<0.05) ,and also increased significantly at 24th hour after SE (t=14.521 ,P<0.05 ;t=19.458 ,P<0.05) .When comparing model group Ⅱ with model group Ⅰ ,LPS pretreatment could significantly increase TLR4 gene level in hippocampus at 3th hour after SE(t=2.362 ,P<0.05)and TLR4 protein expression level in hippocampus at 3th hour after SE(t=4.284 ,P<0.05) ,and the P-IκB-αprotein expression level in hippocampus at 3th and 24th hour after SE also significantly increased(t=4.249 , P<0.05 ;t=9.120 ,P<0.05) ,but there were no significant change of HMGB1 gene level in hippocampus at 3th and 24th hour af-ter SE(t=0.569 ,P>0.05 ;t=0.691 ,P>0.05) ,nor HMGB1 protein expression level in hippocampus at 3th and 24th hour after SE(t=0.168 ,P>0.05 ;t=0.385 ,P>0.05) .Conclusion Infant rats seizures and expression of TLR4/P-IκB-αincreased by LPS pretreatment ,without significant change of HMGB1 expression .关键词
脂多糖/海人酸/癫痫/高迁移率族蛋白1/Toll样受体4/P-IκB-α/大鼠Key words
Lipopolysaccharide/Kainic acid/Epilepsy/High mobility group protein 1/Toll-like receptor 4/P-IκB-α/Rat分类
医药卫生引用本文复制引用
禚志红,张静,李国栋,王怀立..腹腔注射脂多糖对幼鼠癫痫发作及海马TLR4/HMGB1/P-IκB-α表达的影响[J].中国实用神经疾病杂志,2018,21(2):117-123,7.基金项目
河南省高等学校重点科研项目计划(13A320433) （13A320433）
郑州大学第一附属医院"院内青年创新基金计划项目" （）
