中国实用神经疾病杂志2018，Vol.21Issue(3)：239-242,4.DOI:10.12083/SYSJ.2018.03.062
U87胶质瘤裸鼠皮下移植瘤模型建立
Establishment of subcutaneous transplantation model of U 87 Glioma in BALB/c nude mice
摘要
Abstract
Objective To establish subcutaneous transplantation model of U 87 glioma in BALB/c nude mice and monitor the change of tumor's temperature .Methods Ten female BALB/c nude mice were inoculated subcutaneously which was on the back near right thigh of mice with tumor cells of the U87 glioma cell line .Subcutaneous transplantation model of U87 glioma in BALB/c nude mice were established .The volume of tumor was measured with a Vernier caliper .The growth lines of tumor were drawn .Using the infrared imaging to observe the growth of glioma and monitor the change of tumor's temperature .The nude mice were killed on 36 days after the beginning of the inoculation .HE staining was performed to observe the morphologic properties of tumor and immunohistochemically staining was performed to detect the positive expression of GFAP in tumor sites .Results The Establishment of neoplasm transplantation models of U 87 glioma in BALB/c mice were successfully .The pathology of tumor was accord with the morphological features of U 87 glioma cell .The expression of GFAP protein was positive in glioma tissues of tumor .At the early growth of tumor ,the temperature of tumor increased gradually with the development of time .The temperature of tumor had reached the highest on 15 days after the beginning of the experiment .While in the late of tumor growth ,the tempera-ture of tumor decreased with the development of time .Compared the 36 days with 6 days after the beginning of the experiment ,the of temperature of tumor had shown significance(P<0.05) .Conclusion Establishment of neoplasm transplantation models of U 87 glioma in BALB/c mice is successfully .This animal model will be widely used in research of glioma treatment .关键词
神经胶质瘤/裸鼠/动物模型/红外成像/皮下移植/温度/胶质纤维酸性蛋白(GFAP)Key words
Glioma/BALB/C-nu mice/Animal models/Infrared imaging/Subcutaneous transplantation/Temperature/GFAP分类
医药卫生引用本文复制引用
汪柳旭,朱凤军,廖建湘,段婧,陈乾,宋建明..U87胶质瘤裸鼠皮下移植瘤模型建立[J].中国实用神经疾病杂志,2018,21(3):239-242,4.基金项目
深圳市基础研究计划(20130310233932) （20130310233932）
