中国实用神经疾病杂志2018，Vol.21Issue(3)：243-246,4.DOI:10.12083/SYSJ.2018.03.063
阿托伐他汀对局灶性脑缺血大鼠MMP-2 occludin mRNA的影响
Impact of atorvastat on MMP-2 and occludin mRNA in focal cerebral ischemia rats
摘要
Abstract
Objective To observe the changes of IgG ,MMP-2 ,occludin mRNA ,and to investigate the impact of atorvastatin on IgG ,MMP-2 ,occludin mRNA of focal cerebral ischemia rats .Methods One hundred and eight male SD rats were randomly di-vided into sham operation group ,model group and treatment group ,the focal cerebral ischemia model was induced by left middle cerebral artery occlusion ,the observing time was 1d ,3d ,7d after operation .The treatment group received a gavage of atorvastatin 4mg/(kg · d) ,others were given the same amount of normal saline at corresponding time .The changes of IgG ,MMP-2 ,occludin mRNA in each group were observed at each observing time to assess the changes in the blood-brain barrier .The expression of IgG , MMP-2 was analyzed by immunohistochemistry ,the expression of occludin mRNA was analyzed by RT-PCR .Results Compared with the sham operation group ,IgG ,MMP-2 in both model group and treatment group increased at each observing time ,but occlu-din mRNA expression decreased at each observing time ,all of them reached the peak at 3d ,the differences were all statistically sig-nificant (P<0.05) .Compared with the model group ,IgG ,MMP-2 of treatment group decreased at each observing time ,but occlu-din mRNA expression increased at each observing time ,the differences were all statistically significant (P<0.05) .Conclusion The permeability of blood-brain barrier in focal cerebral ischemia rats was increased ,and reached the peak at 3d;atorvastatin can inhibit the activity of MMP-2 ,and increase the expression of occludin mRNA to reduce the permeability of blood-brain barrier and protect brain tissue .关键词
阿托伐他汀/局灶性脑缺血/血脑屏障/MMP-2/occludinmRNAKey words
Atorvastatin/Focal cerebral ischemia/Blood-brain barrier/Occludin mRNA/MMP-2分类
医药卫生引用本文复制引用
杨会杰,张娟,夏昱,孙环,孟祥光..阿托伐他汀对局灶性脑缺血大鼠MMP-2 occludin mRNA的影响[J].中国实用神经疾病杂志,2018,21(3):243-246,4.基金项目
中国博士后科学基金第56批面上资助项目,编号:2014M562001 （）
