中国实用神经疾病杂志2018，Vol.21Issue(4)：349-352,4.DOI:10.12083/SYSJ.2018.04.089
大鼠脑创伤模型中Leptin蛋白表达的时间依从性变化
Correlation between changes of Leptin in experimental traumatic brain injury in rats model
摘要
Abstract
Objective To investigate time-dependentchanges in Leptin concentrations in brain tissue followingexperimental traumatic brain injury and to examine the relationshipwith cytokines such as IL-6 ,IL-1β,TNF-α.Methods The model of SD rat brain contusion was established byfree fall striking method ,and the random digital table was used to randomize the group .Rats weredivided into 3 groups based on thedepth of deformation :sham controls ,moderate injury and severe injury .Animalswere sacri-ficed on the 1st ,3rd and 5th days post-injury .The expression of IL-1β,IL-6 ,TNF-αand Leptin in different time was observed by enzyme-linked immunosorbentassay (ELISA) .Results One day after moderate injury ,IL-1β,IL-6 ,TNF-αand Leptin levelswere found to be markedly increased in the brain tissue .On the 3rd and 5th days ,the levels decreased to the shamcontrollevels .Follow-ing severe injury ,IL-1β,IL-6 andTNF-αlevels increased in correlation after the 1st day andreached the sham-control levels on the same days .However ,Leptin tissue levels decreased on the 1st and 3rd daysand normalized to the sham-control levels on the 5th day .Conclusion Leptin is decreasedin the early stage of severe injury .Leptin level correlates with degree of severetraumatic brain injury .关键词
创伤性脑损伤/炎症因子/Leptin蛋白/时间依从性/大鼠Key words
Traumatic brain injury/Inflammatory factor/Leptin/Time dependent/Rat分类
医药卫生引用本文复制引用
张婷,宋宇,魏志玄..大鼠脑创伤模型中Leptin蛋白表达的时间依从性变化[J].中国实用神经疾病杂志,2018,21(4):349-352,4.基金项目
河南省基础与前沿技术研究计划项目 ,编号 :122300410330 （）
