中国实用神经疾病杂志2018，Vol.21Issue(4)：357-360,4.DOI:10.12083/SYSJ.2018.04.091
硫辛酸对大鼠脑缺血再灌注损伤的神经保护作用
Neuroprotective effects of lipoic acid against focal cerebral ischemia reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effect of lipoic acid on focal cerebral ischemia reperfusion-induced injury in rats and further exploring the underlying mechanisms .Methods Fifty-four male clean SD rats were divided into three groups by the random number method :sham operation group (SO group ,n=18) ,cerebral ischemia-reperfusion group (I/R group ,n=18) , cerebral ischemia-reperfusion group plus lipoic acid treatment group (treatment group ,n=18) .Rats were subjected to 2h of tran-sient middle cerebral artery occlusion (MCAO) ,followed by 24h of reperfusion .Lipoic acid (20 mg/kg) was administered in 18 animals (treatment group) through the external jugular vein immediately after reperfusion .An equivalent volume of vehicle was administered to 36 animals (SO group and I/R group) .The infarct volume of animals was determined by the 2 ,3 ,5-triphenyltet-razolium chloride staining .The expression level of TNF-α was detected by RT-PCR and the apoptotsis was detected by TUNEL method .Results Compared to SO group ,cerebral infarction area ,neuronal apoptosis and cerebral TNF-α production were enhanced significantly in I/R and treatment group .Compared to I/R ,lipoic acid reduced cerebral infarction area ,neuronal apoptosis and cerebral TNF-αproduction .Conclusion Our results suggested that lipoic acid has protective effects against cerebral I/R injury in rats ,which may be attributed to attenuating inflammation and apoptosis induced by cerebral ischemia .关键词
硫辛酸/脑缺血再灌注/梗死体积/肿瘤坏死因子a/凋亡细胞Key words
Lipoic Acid/Ischemia-reperfusion/Infarct volume/TNF-α/Apoptosis分类
医药卫生引用本文复制引用
刘庆杰,王伟,白宏英..硫辛酸对大鼠脑缺血再灌注损伤的神经保护作用[J].中国实用神经疾病杂志,2018,21(4):357-360,4.基金项目
河南省医学教育研究课题 ,编号 :Wjlx2016152 （）
