中国实用神经疾病杂志2018，Vol.21Issue(5)：470-474,5.DOI:10.12083/SYSJ.2018.05.121
脑源性神经生长因子对缺糖缺氧小鼠伏隔核胶质纤维酸性蛋白的影响
The effect of BDNF on GFAP in NAc slices of mice with oxygen-glucose deprivation
喻晓路 1林奇泗 2傅慧3
作者信息
- 1. 徐州医科大学江苏省麻醉学重点实验室,江苏徐州221004
- 2. 徐州医科大学药学院,江苏徐州221004
- 3. 淄博市第一医院神经内一科 ,山东 淄博 255200
- 折叠
摘要
Abstract
Objective To explore the effect of BDNF on GFAP in NAc slices under the condition of oxygen-glucose depriva-tion .Methods 18 mice were randomly divided into normal group (Culture of low-glucose DMEM Medium in NAc slices) ,OGD/R group (culture of EBSS medium 15 minutes after reperfusion 1 hour in NAc slices) and OGD/R+BDNF group (culture of EBSS medium adding BDNF 15 minutes after reperfusion 1 hour in NAc slices) .After treatment ,the expression of GFAP protein was determined .Results The relative expression of GFAP in normal group was 0.544 ± 0.053 ,and the relative expression of GFAP in hypoxia-reperfusion group was 1.439 ± 0.325153 ,the relative expression of GFAP in the drug-deficient hypoxia-reperfusion group was 0.739 ± 0.121 .There was a statistically significant difference in the relative expression of GFAP protein in the OGD /R group compared with the normal group (P<0.01) ,and there was a statistically significant decreased in the protein relative expression of GFAP in the OGD/R+BDNF group and OGD/R group(P<0.05) .There was no statistically significant difference in GFAP pro-tein relative expression between OGD/R+BDNF group and normal group .Conclusion GFAP in the NAc brain region was signifi-cantly elevated under the condition of oxygen-glucose deprivation ,and BDNF inhibits astrocytes .关键词
脑源性神经生长因子(BDNF)/伏隔核(NAc)/缺糖缺氧/胶质纤维酸性蛋白(GFAP)/蛋白表达/小鼠Key words
BDNF/NAc/oxygen-glucose deprivation/GFAP/Protein expression/Mice分类
医药卫生引用本文复制引用
喻晓路,林奇泗,傅慧..脑源性神经生长因子对缺糖缺氧小鼠伏隔核胶质纤维酸性蛋白的影响[J].中国实用神经疾病杂志,2018,21(5):470-474,5.
