基础医学与临床2018，Vol.38Issue(3)：330-334,5.
miR-143靶向调控ER-α36影响胃癌细胞系SGC7901的侵袭
Effects of miR-143 target-regulating ER-α36 on the invasion of gastric cancer cell line SGC 7901
摘要
Abstract
Objective To investigate the effects of ER-α36 on invasion of human gastric cancer cell lines SGC7901 by miR-143. Methods Lentiviral vectors were constructed to generate up-and down-regulations of miR-143 lentiviruses (LV-miR-143 and LV-anti-miR-143,respectively).The viruses were used to infect human gastric cancer cell lines SGC7901.The ER-α36 protein expression level and the invasion of constructed cells were detected by Western blot and transwell. The target gene of miR-143 was predicted by bioinformatics tools.Luciferase reporter assay was carried out to confirm the predicted target gene. Results The infection efficiency of the lentivirus titers of LV-miR-143 and LV-anti-miR-143 were over 80% shown by the green fluorescence. The ER-α36 expression level,the cell invasion in LV-miR-21 group were significantly lower than those in LV-anti-miR-21 group(P<0.05). Conclusions miR-143 plays an important role in the negative control of gastric cancer invasion by the regulation of ER-α36.关键词
胃癌/雌激素受体-α36/细胞侵袭/miR-143Key words
gastric cancer/ER-α36/cell invasion/miR-143分类
医药卫生引用本文复制引用
邹丰,罗媛媛,刘丽江..miR-143靶向调控ER-α36影响胃癌细胞系SGC7901的侵袭[J].基础医学与临床,2018,38(3):330-334,5.基金项目
国家自然科学基金(81272754) （81272754）
武汉市青年科技晨光计划(2013071004010475) （2013071004010475）
