基础医学与临床2018，Vol.38Issue(3)：370-374,5.
转染胃动蛋白2基因抑制人胃癌细胞系MKN28增殖、迁移和侵袭
Gastrokines 2 transfection inhibits proliferation, migration and invasion of gastric cancer cell line MKN28
摘要
Abstract
Objective Gastrokines 2(GKN2) protein expression is detected in gastric cancer tissues, the distal gastric mucosa tissues and the adjacent normal gastric tissues; the effect of GKN2 overexpression is displayed on proliferation,migration and invasion in human gastric cancer cell line MKN28. Methods Immunohistochemistry was performed to observe GKN2 expression in gastric cancinoma tissues,adjacent gastric mucosa tissues and distal gastric mucosa tissues. Then,the GKN2 gene eukaryotic expression vector Xhol GKN3SP-hGKN2-TEV-SBP Xhol was transfected into human gastric cancinoma MKN28 cells. Western blot was used to evaluate the expression of GKN2 in MKN28 cells. Cell viability,migration and invasion ability were investigated by MTT,Transwell migra-tion assay and Transwell invasion assay. Results GKN2 expression in gastric cancinoma tissues is lower in com-parison with those in the adjacent normal gastric tissues or distal gastric mucosa tissues respectively (P< 0.05).The viability of MKN28 cells was significantly inhibited after transfected by GKN2 vs untransfected cells (P<0.05). GKN2 overexpression decreased the cells' number passing through both the membrane and matrigel, compared to those of untransfected cells(P < 0.05). Conclusions Downregulation of GKN2 protein expression is associated with the occurrence of gastric cancer; GKN2 overexpression can suppress the prolifera-tion, migration and invasion of MKN28 cells.关键词
胃肿瘤/胃动蛋白2/增殖/迁移/侵袭Key words
stomach neoplasms/gastrokine 2/proliferation/migration/invasion分类
医药卫生引用本文复制引用
蔡志宏,曹怡静,刘姣,胡皓彬,谢鑫,许雄峰,凌晖..转染胃动蛋白2基因抑制人胃癌细胞系MKN28增殖、迁移和侵袭[J].基础医学与临床,2018,38(3):370-374,5.基金项目
湖南省教育厅科学研究项目(16C1398) （16C1398）
湖南省十二五重点学科"基础医学"资助项目[2011(76)] （76）
肿瘤细胞与分子病理学湖南省重点实验室资助项目(2016TP1015) （2016TP1015）
湖南省(南华大学)研究生科研创新项目(2016XCX37) （南华大学）
