基础医学与临床2018，Vol.38Issue(4)：480-484,5.
羟基红花黄色素A减轻大鼠心肌细胞缺氧/复氧损伤
Hydroxysafflor yellow A reduces anoxia/reoxygenation-induced injury in rat cardiomyocytes
摘要
Abstract
Objective To observe the protective effect of hydroxysafflor yellow A(HSYA) on anoxia/reoxygenation (A/R) injury of neonatal primary cardiomyocytes, and its relationship with phosphoinositide 3-kinase/protein ki-nase B/glycogen synthase kinase 3β(PI3K/Akt/GSK3β) signaling pathway. Methods Primary cardiomyocytes of neonatal rats were isolated from the rats and incubated for 48 hours. The cells were adhered to each other and then divided into five groups:control group (Con group), anoxia/reoxygenation group (A/R group),HSYA treatment group(A/R+H group),PI3K inhibitor (LY294002)treatment group(A/R+L group)and HSYA+LY294002 treat-ment group (A/R+H+L group),then to collect the supernatant fluid of each group to measure LDH.The flow cy-tometry was used to measure the apoptotic cells. The protein levels of Bcl-2,Bax,Akt,p-Akt (Ser473),GSK3β, p-GSK3β (Ser9) were evalated by Western blot. Results A/R increased LDH release,the apoptosis rate (P<0.001),and the expression of pro-apoptotic protein Bax (P <0.001) with the decrease of anti-apoptotic protein Bcl-2,p-Akt(Ser473), p-GSK3β(Ser9)(P<0.001) as compared with the control group. HSYA treatment de-creased LDH release,the apoptosis rate (P<0.001),and the expression of Bax (P<0.001) and increase the ex-pression of Bcl-2,p-Akt(Ser473),p-GSK3β(Ser9)(P<0.001). Compared with the A/R+H group,the expres-sion of Bax was increased (P<0.001),while the expression of Bcl-2, p-Akt(Ser473), p-GSK3β(Ser9)was de-creased (P<0.001) in the A/R+H+L group. Conclusions HSYA protects rats'cardiomyocytes from anoxia/reoxy-genation injury by regulating PI3K/Akt/GSK3β signaling pathway.关键词
缺氧/复氧/HSYA/心肌细胞/凋亡/PI3K/Akt/GSK3β信号通路Key words
anoxia/reoxygenation/HSYA/cardiomyocytes/apoptosis/PI3K/Akt/GSK3β signaling pathway分类
医药卫生引用本文复制引用
沈冰冰,张松,朱启仁,王志荣,张卓琦..羟基红花黄色素A减轻大鼠心肌细胞缺氧/复氧损伤[J].基础医学与临床,2018,38(4):480-484,5.基金项目
国家自然科学基金(30800219) （30800219）
