基础医学与临床2018，Vol.38Issue(4)：543-547,5.
CRISPR/Cas9基因编辑系统研究进展
Advances in CRISPR/Cas9 gene-editing system
摘要
Abstract
CRISPR/Cas9 gene-editing system has been broadly used in various fields of bioscience and medicine in recent years. The system can be guided by RNA to specific DNA site thus achieving targeted gene editing.Off-target effect and editing efficiency remain to be two crucial challeges to the system. Currently, a number of researches have been focused on the optimization of the system by reducing off-target effects and increasing editing efficiency, which may enhance its safety and expand its application.关键词
CRISPR/Cas9/基因编辑/脱靶效应/编辑效率Key words
CRISPR/Cas9/gene editing/off-target effect/editing efficiency分类
生物科学引用本文复制引用
程成,舒鹏程,彭小忠..CRISPR/Cas9基因编辑系统研究进展[J].基础医学与临床,2018,38(4):543-547,5.基金项目
国家重点研发计划 (2016YFA0100702,2016YFC0902502) （2016YFA0100702,2016YFC0902502）
国家重点基础研究发展计划 (2013CB531304) （2013CB531304）
国家自然科学基金 (31301152, 31370789, 31670789, 31671316) （31301152, 31370789, 31670789, 31671316）
中国医学科学院(CIFMS, 2016-I2M-2-001, 2016-I2M-1-001,2016-I2M-1-004) （CIFMS, 2016-I2M-2-001, 2016-I2M-1-001,2016-I2M-1-004）
