激光生物学报2018，Vol.27Issue(1)：28-35,8.DOI:10.3969/j.issn.1007-7146.2018.01.005
三角褐指藻光合与生长参数测量方法的研究
A Study on Measurement Methods of Photosynthesis and Growth Parameters of Phaeodactylum tricornutum
摘要
Abstract
Phaeodactylum tricornutum is an excellent microalgae of the biodiesel production,it is obviously necessary to monitor the photosynthesis and growth parameters of microalgae through the culture process. In this study,the cell densi-ty was monitored by cell count,and chlorophyll fluorescence parametes,nitrate content in the culture medium,cell chlo-rophyll fluorescence intensity and neutral fat relative content were detected by using the MultiColor PAM,NanoDrop UV spectrophotometer and fluorescence spectrophotometer, respectively. The results showed that the number of cells and chlorophyll fluorescence intensity had a linear relationship only at the growth phase,after reaching the stationary phase, the chlorophyll content significantly decreased,when chlorophyll fluorescence intensity was not proportional to cell densi-ty. The maximum photochemical quantum efficiency Fv/Fmof PSII which showed two ascending and descending proces-ses in the culture stage was positively correlated with the specific growth rate of chlorophyll fluorescence intensity. The photochemical quenching coefficient qL increased in the culture process, while the relative electron transport rate rETR and the actual photosynthetic efficiency Y(II) both showed the trend of rising first and then down. The content of nitrate in the culture medium decreased to a certain extent,and then no longer reduced. The cell neutral fat relative content de-creased after 1 day of cell inoculation and then continued to increase after the nitrate content did not decrease any more. In this study, the correlation data of photosynthesis and growth parameters, and the establishment of the method for measuring nitrate content in the culture medium and cell neutral fat relative content,can provide the necessary detection methods and research foundation for the laboratory culture and metabolite accumulation of P. tricornutum.关键词
三角褐指藻/细胞密度/叶绿素荧光强度/叶绿素荧光参数/硝酸盐含量/中性脂相对含量Key words
Phaeodactylum tricornutum/cell density/chlorophyll fluorescence intensity/chlorophyll fluorescence pa-rameter/nitrate content/neutral fat relative content分类
农业科技引用本文复制引用
陈禹先,佟少明,侯和胜..三角褐指藻光合与生长参数测量方法的研究[J].激光生物学报,2018,27(1):28-35,8.基金项目
辽宁省海洋与渔业厅项目(201410) （201410）
