激光生物学报2018，Vol.27Issue(1)：45-52,8.DOI:10.3969/j.issn.1007-7146.2018.01.007
红鲫Gsk3β基因克隆与分化表达模式
A Study on Cloning and Differential Expression of Gsk3β in Red crucian carp
摘要
Abstract
Objective:To investigate the expression pattern of Gsk3β gene in different developmental stages and adult tissues of red crucian carp, as well as its molecular response under cadmium stress. Methods:The full length of Gsk3β cDNA was obtained by RACE cloning technique,which Gsk3β mRNA and protein was analyzed by semi-quantitative RT-PCR and Western Blot.The expression of Gsk3β was analyzed by specific concentration of cadmium. Results: The total length of Gsk3β gene was 2 195 bp,the 5′untranslated region (UTR) was 692 bp, and the 3′ untranslated region was 237 bp. Encoding 421 amino acids containing protein and protein molecular weight was 46 846.40,its isoelectric point was 9.05,and has the closest relationship with zebrafish and medaka.Gsk3β gene was expressed in different embryonic developmental stages and adult tissues.The expression of Gsk3β was most abundant in the brain tissue, and the expres-sion of Gsk3β in the muscle and testis was higher than in the liver and kidney,ovaries and skin tissues. At different de-velopmental stages, Gsk3β was not expressed in the two-cell stage, and then expressed at different stages of follow-up. The expression of Gsk3β was the strongest in the last stage,followed by the duration of muscle,In the eye pigment period and somatic stage protein expression is weak. The expression of Gsk3β related genes were detected in the liver of red cru-cian carp after cadmium treatment, and the result showed that expression level of Gsk3β was in down-regulation. Con-clusion:Gsk3β gene plays an important role in different embryonic developmental stages of red crucian carp and partici-pates in cadmium stress response, but its specific molecular mechanism remains to be further studied.关键词
Gsk3β/基因克隆/PCR/westernblotKey words
Gsk3β/gene cloning/PCR/western blot分类
生物科学引用本文复制引用
张卉琴,李万程,刘文彬,张永勤,邓美霞,桂赛玉,文苑桧,刘锦辉,陈志男,李晓超,肖亚梅..红鲫Gsk3β基因克隆与分化表达模式[J].激光生物学报,2018,27(1):45-52,8.基金项目
长沙市科技计划项目(kq1701046) （kq1701046）
湖南省科技计划重点研发计划资助项目(2017SK2302,2016NK2046) （2017SK2302,2016NK2046）
2017年度湖南省大学生研究性学习和创新性实验计划项目(2017030) （2017030）
湖南省科技计划资助项目(2014FJ3084) （2014FJ3084）
