检验医学与临床2018，Vol.15Issue(6)：813-816,4.DOI:10.3969/j.issn.1672-9455.2018.06.023
阿司匹林通过调节血管生成相关因子抑制非小细胞肺癌增殖及凋亡的机制
Mechanisms of aspirin for inhibiting proliferation and apoptosis of non-small cell carcinoma by regulating angiogenesis-related factors
顾宪文 1谷兰海 1邢加强 1李殿波 1于海防1
作者信息
- 1. 山东省临沂市肿瘤医院胸外科 276000
- 折叠
摘要
Abstract
Objective To investigate the mechanism of aspirin for inhibiting the proliferation and apoptosis of non-small cell carcinoma(A549)by regulating angiogenesis-related factors.Methods The A549 cells were cultured in vitro to the logarithmic phase and intervened with aspirin at different level gradients(0.0,2.5,5.0, 10.0,20.0 mmol/L).The effect of different aspirin concentration gradients on the cell proliferation rate was detected by using the thiazolyl blue(M T T)method.T he A549 cell apoptosis rates at different cellular cycles were detected by using the flow cytometry.The transcriptional level of vascular endothelial growth factor (VEGF),epidermal growth factor receptor(EGFR)and fibroblast growth factor(FGF)were detected by Western blotting.Results After aspirin acting on A549 cells,the inverted microscope observed the changes of irregular cellular morphology,increased permeability and cellular disintegration and broken.T he M T T results showed that the aspirin action on A549 cells could inhibit cell proliferation in a time and dose dependent man-ner.Aspirin could induce apoptosis of A 549 cells in S phase and block the proliferation of A 549 cells in G0/G1 phase and G2/M phase.The expression levels of VEGF,EGFR and FGF after aspirin acting on A549 cells were significantly decreased in a dose-dependent manner.Conclusion Aspirin can inhibit the in vitro prolifera-tion of non-small cell carcinoma A549 cells and induces the cellular apoptosis in S phase.The apoptosis mecha-nism may be related to the inhibition of angiogenesis-related factors by aspirin.关键词
血管内皮生长因子/表皮生长因子受体/成纤维细胞生长因子/阿司匹林/非小细胞肺癌Key words
angiogenesis-related factor/non-small cell carcinoma/proliferation/aspirin/apoptosis分类
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顾宪文,谷兰海,邢加强,李殿波,于海防..阿司匹林通过调节血管生成相关因子抑制非小细胞肺癌增殖及凋亡的机制[J].检验医学与临床,2018,15(6):813-816,4.
