兰州大学学报（医学版）2018，Vol.44Issue(1)：53-58,6.DOI:10.13885/j.issn.1000-2812.2018.01.010
饥饿诱导K562/ADM细胞自噬及其与凋亡的关系
Relationship between the changes in autophagic activity and apoptosis of K562/ADM cells induced by starvation
摘要
Abstract
Objective To investigate the relationship between the changes in autophagic activity and apoptosis of K562/ADM cells induced by starvation.Methods Human leukemia K562/ADM cells were induced by starvation (3 h,6 h and 9 h) in vitro.Autophagic vacuoles were observed by electron microscope,the apoptotic rates and the increased MDC (monodansylcadaverin)-positive cells of K562/ADM were detected with flow cytometry,the expression of LC3 mRNA,Beclin-1 mRNA and Bcl-2 mRNA were detected by real-time quantitative PCR (qPCR),the expression of LC3,Beclin-1,Bcl-2 protein and the result of LC3-I converted to LC3-Ⅱ were detected by Western blot.Results Starvation could induce obvious changes in the autophagic activity of K562/ADM cells after their being starved 3 h,obvious autophagic vacuoles were observed under electron microscope,the increased expression of LC3 gene mRNA and LC3-Ⅱ protein was detected,but autophagic activity of K562/ADM cells decreased with prolonged starvation.Starvation could induce the increased apoptosis in a time-dependent manner;the expression of Beclin-1 in starved K562/ADM was positively correlated with the changes of autophagic activity but negatively correlated with apoptosis,while the expression of Bcl-2 was opposite.Conclusion Starvation could induce changes in the autophagic activity,which may protect against apoptosis induced by starvation in K562/ADM cells,and Beclin-1 and Bcl-2 may be involved in the molecular mechanisms.关键词
K562/ADM细胞/自噬/凋亡Key words
K562/ADM cells/autophagy/apoptosis分类
医药卫生引用本文复制引用
成娟,薛明明,马海珍,张豪,赵龙..饥饿诱导K562/ADM细胞自噬及其与凋亡的关系[J].兰州大学学报（医学版）,2018,44(1):53-58,6.基金项目
甘肃省普通中医药科研项目(甘肃省中医药管理局GZK-2012-14) （甘肃省中医药管理局GZK-2012-14）
