兰州大学学报（医学版）2018，Vol.44Issue(1)：59-64,6.DOI:10.13885/j.issn.1000-2812.2018.01.011
uPAR衍生RERF肽逆转K562/ADM耐药及其机制
Effect and mechanism of uPAR-derived peptide in reversing multi-drug resistance in human leukemia K562/ADM cells
摘要
Abstract
Objective To investigate its effect of the newly synthesized uPAR-derived peptide (RERF peptide) on leukemic K562/ADM and its mechanism.Methods The effect of RERF peptide on the proliferation of K562/ADM cells was observed by MTT assay.The reversal effect of non-cytotoxic dose of RERF peptide on drug resistance of adriamycin resistant K562/ADM cells was observed.The relative expression of uPA,uPAR and MDR1 gene in K562/ADM cells was detected by qPCR.And the expression of uPA,uPAR and P-gp protein in K562/ADM cells were detected by a western blot analysis.Results The IC50 values of K562/ADM cells were 92.48±0.27,30.99±0.19,28.06±0.12 μg/mL respectively at 24 h,48 h,and 72 h after ADM administration.The IC50 decreased to 83.83±0.29,18.52±0.17,16.38±0.13 μg/mL respectively at 24 h,48 h and 72 h after ADM combined with non-cytotoxic doses of RERF peptide.The expression of uPA,uPAR,MDR1 mRNA and p-gp protein in K562/ADM cells after treatment 48 h were significantly decreased (P ＜ 0.05).Conclusion RERF peptide could partially reverse the drug resistance of ADM in K562/ADM cells and improve the sensitivity of ADM.RERF peptide could reverse the resistance by affecting MDR1 gene via the uPA/uPAR pathway and decrease the express of P-gp.关键词
尿激酶型纤溶酶原激活物受体/RERF肽/多药耐药Key words
urokinase-type plasminogen activator receptor/RERF peotide/multi-drug resistance分类
医药卫生引用本文复制引用
郭鸿,张淑玲,周兰霞,马云云,贾芳,胡新新,张慧,赵丽..uPAR衍生RERF肽逆转K562/ADM耐药及其机制[J].兰州大学学报（医学版）,2018,44(1):59-64,6.基金项目
甘肃省卫生行业项目(GWGL2013-23) （GWGL2013-23）
