兰州大学学报（医学版）2018，Vol.44Issue(1)：65-70,6.DOI:10.13885/j.issn.1000-2812.2018.01.012
莪术醇诱导胆管癌细胞凋亡和调控细胞周期的体外研究
The Study on the apoptosis effect and cell cycle regulation of curcumol on cholangiacarcinoma cell line in vitro
摘要
Abstract
Objective To observe the effect of curcumol on the proliferation and apoptosis of intrahepatic cholangiocarcinoma RBE cells in vitro and explore its possible mechanisms.Methods The inhibitory effect of curcumol on RBE cells was estimated with XTT assay,and different action times (24,48,72 h) and curcumol concertrations (12.5,25,50,75,100 μg/mL) were adopted.Caspase-3/7 activity was determined by microplate reader.Alteration of cell cycle and apoptosis rate were detected by flow cytometry and the expression of Cyclin D1,NF-κB,and Bcl-2 were measured with Western Blot.Results Curcumol had remarkably inhibitory effects on the proliferation of RBE cells and was in a concentration-and time-dependent manner within certain range (50-100 μg/mL).After treatment with curcumol for 48 hours,the activity levels of Caspase-3/7 significantly increased and this could induce RBE cells arrest in G1 phase with the expression level of Cyclin D1 being increased,while the NF-κB,Bcl-2 levels were decreased.Conclusion Curcumol could moderately inhibit the proliferation of RBE cells and induce G1 arrest of cell cycle and induce cell apoptosis via mitochondrial pathways.关键词
莪术醇/胆管癌/细胞周期/凋亡Key words
curcumol/cholangiocarcinoma/cell cycle/apoptosis分类
医药卫生引用本文复制引用
张金铎,苏刚,逯娅雯,周昕玥,赵盛,把永江,唐增伟,孟文勃,李汛..莪术醇诱导胆管癌细胞凋亡和调控细胞周期的体外研究[J].兰州大学学报（医学版）,2018,44(1):65-70,6.基金项目
“西部之光”人才培养引进计划项目(科发人函字(2015)90号) （科发人函字(2015）
甘肃省中医药管理局科研项目(GZK-2015-69) （GZK-2015-69）
甘肃省自然科学基金项目(17JR5RA273) （17JR5RA273）
