临床肝胆病杂志2018，Vol.34Issue(3)：540-543,4.DOI:10.3969/j.issn.1001-5256.2018.03.018
双氯芬酸钠对经内镜逆行胰胆管造影术后胰腺炎息者非凿体类抗炎药激活基因1水平的影响
Effect of diclofenac sodium on the level of non-steroidal anti-inflammatory drug-activated gene-1 in patients with post-ERCP pancreatitis
胡翠 1鲍峻峻 1刘晓昌 1丁少桢 1梅俏 1许建明1
作者信息
- 1. 安徽医科大学第一附属医院消化内科,消化系统疾病省级重点实验室,合肥230022
- 折叠
摘要
Abstract
Objective To investigate the effect of diclofenac sodium on the level of non-steroidal anti-inflammatory drug-activated gene-1(NAG-1)in patients with post-ERCP pancreatitis(PEP). Methods A total of 120 patients who underwent ERCP in The First Affilia-ted Hospital of Anhui Medical University from September 2012 to October 2013 were enrolled and randomly divided into diclofenac sodium group and control group,with 60 patients in each group. The patients in the diclofenac sodium group were given intramuscular injection of Olfen(containing diclofenac sodium 75 mg)immediately after ERCP. Blood samples were collected before surgery and at 3 and 24 hours af-ter surgery,and the level of amylase was measured. The incidence of abdominal pain was also observed and the incidence rate of PEP was calculated for both groups. RT-PCR and Western blot were used to measure the mRNA and protein expression of NAG-1 in plasma. An repeated-measures analysis of variance was used for comparison of continuous data;a univariate analysis of variance was used for data meet-ing the requirements of sphericity test,and the Greenhouse-Geisser correction method was used for data which did not meet the require-ments of sphericity test. The chi-square test was used for comparison of categorical data between groups. Results The diclofenac sodium group had a significantly lower incidence rate of PEP than the control group[6.67%(4/60)vs 20.00%(12/60),χ2=4.62,P=0.03]. The diclofenac sodium group had a significantly lower level of amylase than the control group at 3 and 24 hours after surgery(at 3 hours after surgery:202.70 ± 120.44 U/L vs 283.57 ± 178.39 U/L,t=2.06,P<0.05;at 24 hours after surgery:209.13 ± 157.14 U/L vs 305.97 ± 208.69 U/L,t=2.03,P<0.05). At 3 hours after ERCP,the diclofenac sodium group had significant increases in the mRNA and protein expression of NAG-1 in plasma and significantly higher mRNA and protein expression of NAG-1 than the control group(all P<0.01). Conclusion Diclofenac sodium can prevent PEP,possibly by promoting the level of NAG-1.关键词
胰腺炎/胰胆管造影术,内窥镜逆行/二氯芬酸/非甾体类抗炎药激活基因1Key words
pancreatitis/cholangiopancreatography,endoscopic retrograde/diclofenac/NSAID activated gene 1分类
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胡翠,鲍峻峻,刘晓昌,丁少桢,梅俏,许建明..双氯芬酸钠对经内镜逆行胰胆管造影术后胰腺炎息者非凿体类抗炎药激活基因1水平的影响[J].临床肝胆病杂志,2018,34(3):540-543,4.
