临床肝胆病杂志2018，Vol.34Issue(3)：588-590,3.DOI:10.3969/j.issn.1001-5256.2018.03.028
裸鼠皮下泡型棘球蝴病移植瘤模型的建立
Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice
摘要
Abstract
Objective To establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice,and to lay a foundation for further research on alveolar echinococcosis. Methods A total of 25 nude mice were given subcutaneous injection of 0.1 ml 20% Echi-nococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded,and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. Results Subcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model,the survival rate of the nude mice was 92%(23/25),and the infection rate of Echinococcus multilocularis was 100%(23/23). A small number of protoscolices died and were blue on trypan blue stai-ning,and the cell viability of protoscolices was 98%. Conclusion Echinococcus multilocularis can be successfully transplanted to the subcu-taneous part of nude mice,which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.关键词
棘球蚴病,肝/疾病模型,动物Key words
echinococcosis,hepatic/disease models/animal分类
医药卫生引用本文复制引用
周启锋,张灵强,廖梦娇,庞明泉,李梦轩,任利,樊海宁..裸鼠皮下泡型棘球蝴病移植瘤模型的建立[J].临床肝胆病杂志,2018,34(3):588-590,3.基金项目
青海大学附属医院中青年科研基金重点项目(ASRF -2016-ZD-02) （ASRF -2016-ZD-02）
青海省科技计划项目(2014-ZJ-719) （2014-ZJ-719）
