临床口腔医学杂志2018，Vol.34Issue(3)：131-134,4.DOI:10.3969/j.issn.1003-1634.2018.03.001
基因重组人生长激素对骨髓基质细胞增殖与成骨性分化的影响
Effects of recombinant human growth hormone on the proliferation and differentiation of bone marrow stromal cells
摘要
Abstract
Objective:To study the effect of different concentrations of recombinant human growth hormone(rhGH) on the proliferation and osteogenic differentiation of bone marrow stromal cells cultured in vitro.Methods:Bone marrow stro-mal cells were isolated from rat bone marrow and cultured in vitro.Different concentrations of rhGH(0,2,5,25,50,150,500 μg/L)were added to observe the cell proliferation rate and detect the activity of intracellular alkaline phosphatase(ALP). Bone marrow stromal cells were cultured with osteogenesis induced in vitro,and the mRNA expressions of ALP,CoL-1 and OCN were detected by RT-PCR.Results:Different concentrations of rhGH could stimulate the growth of bone marrow stromal cells,enhance the activity of ALP and improve the expression of ALP,Coll,OCN mRNA in vitro.The mRNA expression of ALP,CoL-1,OCN,the activity of ALP and the proliferation rate was significantly increased at 150μg/L.Conclusion:Differ-ent concentrations of rhGH can potentiate the proliferation and differentiation of bone marrow stromal cells in vitro,and it has the most significant effect at the concentration of 150μg/L.关键词
基因重组人生长激素/骨髓基质细胞/骨性分化Key words
rhGH/Bone marrow stromal cells/Osteogenesis分类
生物科学引用本文复制引用
朱怀安,尹苗,陈婉丽,铁朝荣..基因重组人生长激素对骨髓基质细胞增殖与成骨性分化的影响[J].临床口腔医学杂志,2018,34(3):131-134,4.基金项目
国家自然科学基金青年科学基金项目(81500899)湖北省自然科学基金资助项目(220100321) （81500899）
