临床口腔医学杂志2018，Vol.34Issue(3)：138-140,3.DOI:10.3969/j.issn.1003-1634.2018.03.003
正畸对牙周膜细胞OPG、RANKL表达的影响
The effects of orthodontic force on the expression of OPG and RANKL in human periodontal ligament cells during orthodontic tooth movement
摘要
Abstract
Objective:To investigate the effect of orthodontic force on the periodontal ligament metabolism of mobile tooth and the role of periodontal ligament metabolism in the bone remodeling during orthodontics.Methods:The cases of or-thodontic tooth extraction were selected.After the removal of the first premolar,the human periodontal ligament cells were harvested from both the pressure side and tension side of the canines in three time including before the traction movement of the canine,three months of the traction movement,and after the traction movement.The expressions of osteoprotegerin(OPG) and nuclear factor-κB ligand(RANKL)were detected by RT-PCR.Results:Human periodontal ligament cells were success-fully cultured in primary culture.Similar results were observed of OPG expression in the tension side and RANKL expression in the pressure side,which was negative before the canine movement,the expression was the highest at three months of canine movement and weakened at the end of the canine movement,and the difference was significant respectively(P<0.01).Con-clusion:OPG and RANKL exist in periodontal ligament cells.Their activities are enhanced when teeth move during orthodon-tics and they may participate in the whole process of orthodontic tooth movement and local alveolar remodeling.关键词
正畸/牙齿移动/人牙周膜细胞/护骨素/细胞核因子-κB受体活化因子配体Key words
Orthodontics/Tooth movement/Human periodontal ligament cells/Osteoprotegerin(OPG)/Receptor-ac-tivator of nuclear factor-κ B ligand(RANKL)分类
医药卫生引用本文复制引用
刘长庚,赵殿才,程自力,聂玉洁..正畸对牙周膜细胞OPG、RANKL表达的影响[J].临床口腔医学杂志,2018,34(3):138-140,3.基金项目
2017年佛山市自筹经费类科技计划项目(医学类科技攻关)(2017AB000182) （医学类科技攻关）
