辽宁农业科学Issue(6)：1-5,5.DOI:10.3969/j.issn.1002-1728.2017.06.001
建立玉米幼胚高效再生体系的研究
Study on the Establishment of High Efficiency Regeneration System of Maize
摘要
Abstract
To establish high-frequency regenration technique system for gene tranformation in maize,We studyed on callus induction rate in L-Pro、AgNO3 and 2,4-D when immature embryo is used as a material;we also analyzed the effects of different concentrations of ZT and IBA hormone on differentiation and rooting,The results showed that the best callus induction rate is added to L-Pro 700 mg/L、AgNO3 0.1 mg/L and 2、4-D1.5 mg/L in basci medium;regeneration buds rate and regeneration plants rate reached 97.96％ and 97.67％ respectively if the basice mediuim was added to 5 mg/L zeatin;The result of rooting was the best when basice meidum was added 0.1 mg/L IBA as the exogenous hormone.关键词
玉米/幼胚/愈伤诱导率/再生Key words
Maize/Immature embryo/Callus-induced rate/Regeneration分类
农业科技引用本文复制引用
李金红,霍岩,付莉,陶承光,史振声..建立玉米幼胚高效再生体系的研究[J].辽宁农业科学,2017,(6):1-5,5.基金项目
辽宁省企业博士后项目(137301) （137301）
国家现代农业产业技术体系(CARS-06-01-06) （CARS-06-01-06）
科技部其他项目(2016YFD0101803) （2016YFD0101803）
