林业工程学报2018，Vol.3Issue(2)：95-101,7.DOI:10.13360/j.issn.2096-1359.2018.02.016
碱性亚硫酸盐预处理对杂交狼尾草酶水解特性的影响
Effect of alkaline sulfite pretreatment on enzymatic hydrolysis of hybrid China wolftailgrass
摘要
Abstract
With the increase in global energy demand and global warming concerns caused by the consumption of fossil fuels,the use of diverse biomass to produce biofuels is of particular interest. Biomass, such as agricultural residue, grasses,and forestry wastes,is considered to be a viable and sustainable source for biofuels production. Hybrid China wolftailgrass(Pennisetum americanum ×P. purpureum)is an attractive alternative biomass for the production of bio?fuels and biochemicals due to its advantages of fast growth,high biomass contents,and wide environmental adaptabil?ity. In this study,P. americanum×P. purpureum was pretreated with Na2SO3,NaOH and Na2SO3?NaOH to investi?gate the effects of various pretreatments on its chemical compositions and crystallinity index.In addition,the effects of enzyme dosages,the synergistic effects between cellulases and xylanase and the combination of enzyme cocktails on enzymatic digestibility during the hydrolysis process were also investigated for the purpose of decreasing enzyme dos?age and enhancing hydrolysis yield. The results showed that after pretreatments with 1% NaOH or 4% Na2SO3, the contents of lignin in P. americanum × P. purpureum decreased from 19.5% to 10%-15%. The Na2SO3?NaOH pre?treatment achieved low lignin content(<5%)and high carbohydrates content(>95%). Less than 10 U/g loading could be used in enzymatic hydrolysis of Na2SO3?,NaOH?and Na2SO3+NaOH?pretreated P. americanum × P. pur?pureum. Supplementation of xylanase was more effective to increase the hydrolysis yield than increasing cellulases loading. High glucose(94.2%)and xylose(84.0%)yields were obtained after supplementation of low enzyme dosa?ges(cellulases of 10 U/g,β?glucosidase of 1 000 nkat/g,and xylanase of 2 mg/g in dry matter)in the hydrolysis of 10% P. americanum × P. purpureum. The results suggested that alkaline sulfite pretreatment exhibited great potential and efficient enzymatic hydrolysis of P. americanum × P. purpureum with low enzyme dosages.关键词
杂交狼尾草/碱法亚硫酸盐预处理/酶水解/纤维素酶/木聚糖酶Key words
hybrid China wolftailgrass(Pennisetum americanum × P. purpureum)/alkaline sulfite pretreatments/en-zymatic hydrolysis/cellulases/xylanase分类
农业科技引用本文复制引用
王敬枫,郝茜珣,侯新村,张军华..碱性亚硫酸盐预处理对杂交狼尾草酶水解特性的影响[J].林业工程学报,2018,3(2):95-101,7.基金项目
国家自然科学基金(31670598,31270622). （31670598,31270622）
