国际眼科杂志2018，Vol.18Issue(1)：32-34,3.DOI:10.3980/j.issn.1672-5123.2018.1.07
白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制
Effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism
摘要
Abstract
AIM:To investigate effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism.METHODS:Human retinal vascular endothelial cells were cultured in low glucose or high glucose medium,the genomic DNA was extracted and copy number of mitochondrial DNA was detected by real-time PCR.Effects of resveratrol on the mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose medium were studied.The expression of mitochondrial transcription factor A (TFAM) and acetylated proliferator-activated receptor coactivator-1 alpha (PGC-1α) were analyzed by Western blot and coimmunoprecipitation.RESULTS:High glucose inhibited the copy number of mitochondrial DNA in retinal vascular endothelial cells.However,resveratrol decreased the level of acetylated PGC-1α in retinal vascular endothelial cells,increased the expression of TFAM and the copy number of mitochondrial DNA.CONCLUSION:Resveratrol may improve mitochondrial function of retinal vascular endothelial cells exposed to a high glucose environment via activation of the PCG-1α-TFAM signaling pathway.关键词
白藜芦醇/视网膜血管内皮细胞/线粒体DNA/TFAM/PGC-1αKey words
resveratrol/retinal vascular endothelial cells/mitochondrial DNA copy/mitochondrial transcription factor A/proliferator-activated receptor coactivator-1 alpha引用本文复制引用
韦艳,苏晓庆,李红,吴冰..白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制[J].国际眼科杂志,2018,18(1):32-34,3.基金项目
国家青年科学基金(No.81500748)National Science Foundation for Young Scientists of China (No.81500748) （No.81500748）
