南京医科大学学报（自然科学版）2018，Vol.38Issue(2)：155-160,6.DOI:10.7655/NYDXBNS20180203
小鼠PNPLA7敲降重组腺病毒的构建及其生物学功能的初步研究
Construction of recombinant adenovirus carrying mouse PNPLA7 shRNA and Preliminary investigation on the biology function of PNPLA7
摘要
Abstract
Objective:To construct recombinant PNPLA7 shRNA adenovirus and check its knockdown efficiency in liver.Provide a new approach for further investigating the role of PNPLA7 on hepatic triglyceride metabolism.Methods:siNC and siPNPLA7 were designed and cloned into the p-shuttle-CMV-silence vector.After Sequencing identification,the plasmid was linearized by Pme Ⅰ and recombined with backbone pAdeasy in BJ5183 competent cells.The recombinant plasmid was linearized by Pac Ⅰ and transfected into 293A cells for packaging Ad-shPNPLA7 and Ad-shNC adenovirus.After 7 days of tail vein injection,Western blot was applied to determine the knockdown efficiency of pAdeasy-shPNPLA7 adenovirus in liver,and hepatic triglycerides content were measured,Results:The hepatic PNPLA7 protein levels were significantly decreased while the hepatic TAG levels were significantly increased in mice with tail vein injection of pAdeasy-shPNPLA7 adenovirus compared with control mice.Conclusion:The recombinant adenovirus of pAdeasyshPNPLA7 and pAdeasy-shNC were successfully constructed.The PNPLA7 may play a role in hepatic triglycerides metabolism.关键词
PNPLA7/腺病毒/甘油三酯/脂代谢Key words
PNPLA7/adenovirus/triglycerides/lipid metabolism分类
医药卫生引用本文复制引用
雷永强,王秀云,李仲..小鼠PNPLA7敲降重组腺病毒的构建及其生物学功能的初步研究[J].南京医科大学学报（自然科学版）,2018,38(2):155-160,6.基金项目
国家自然科学基金(81471079,31271268) （81471079,31271268）
