南京医科大学学报（自然科学版）2018，Vol.38Issue(2)：185-190,6.DOI:10.7655/NYDXBNS20180208
脂氧素A4对高氧损伤肺泡Ⅱ型细胞系MLE-12的保护作用
Protective effect of lipoxin A4 on hyperoxia injury of type Ⅱ alveolar epithelial cell line MLE-12
摘要
Abstract
Objective:To investigate protectory effects and possible mechanisms of lipoxin A4(LXA4)on the type Ⅱ alveolar epithelial cell line MLE-12 with hyperoxia injury.Methods:MLE-12 cells were cultured and divided into five groups randomly:I:air group,Ⅱ:hyperoxia group,Ⅲ:hyperoxia+ LXA4 group,Ⅳ:hyperoxia+ TGF-β1 neutralizing antibodies,Ⅴ:hyperoxia+ TGF-β1 neutralizing antibodies+ LXA4.The growth state and morphological changes of the cells were observed by the inverted phase microscope;the mRNA expression of collagen Ⅰ,tenascin-C,TGF-β1 receptor(TGF-βR1),TGF-βR2 and Smad3 were measured by real-time quantitative PCR;the protein levels of TGF-ββ1/Smads signaling pathway(TGFβR1,Smad2,Smad3,Smad4,p-Smad2 and p-Smad3)were detected by Western blot.Results:①Morphological change:the cells under hyperoxia lost their normal shapes and showed necrosis,while the cells cultured with LXA4 and/lor TGF-β 1 neutralizing antibodies were normal appropriately.②The mRNA expression of extracellular matrix (ECM):LXA4 and TGF-β1 neutralizing antibodies attenuated the mRNA expression of collagen Ⅰ,tenascin-C markedly (P ＜ 0.05),which expressed in the group hyperoxia+TGF-ββ1 neutralizing antibodies + LXA4 decreased most obviously.③The mRNA and protein levels of TGF-β1/Srnads signaling pathway:Compared with that of the hyperoxia group,the mRNA expression of TGF-βR1,TGF-βR2 and Smad3 decreased(P ＜ 0.05),while the protein level of TGF-β1/Smads signaling pathway had the same change in intervention group,and it in the the group hyperoxia+ TGF-β1 neutralizing antibodies +LXA4 decreased most obviously (P ＜ 0.05).Conclusion:LXA4 can achieve protection of hy-peroxia-induced injury to MLE-12 which may be related to collagen Ⅰ,tenascin-C and TGF-ββ1/Smads signaling pathway.关键词
脂氧素A4/TGF-β1/Smads信号通路/细胞外基质/高氧损伤Key words
lipoxin A4/TGF-β1/Smads signaling pathway/extracellular matrix/hyperoxia injury分类
医药卫生引用本文复制引用
李淑君,李冰洁,罗妍妍,卢红艳,吴升华,陈筱青..脂氧素A4对高氧损伤肺泡Ⅱ型细胞系MLE-12的保护作用[J].南京医科大学学报（自然科学版）,2018,38(2):185-190,6.基金项目
国家自然科学基金资助(81300521,81270821,81370746) （81300521,81270821,81370746）
