南京医科大学学报（自然科学版）2018，Vol.38Issue(2)：201-205,238,6.DOI:10.7655/NYDXBNS20180211
多光子显微成像技术用于诊断结直肠疾病可行性的初步研究
Initial investigation of the feasibility of real-time multiphoton microscopic imaging for diagnosing colorectal diseases
摘要
Abstract
Objective:To investigate and establish the microscopic features of colorectal diseases via multiphoton microscopy (multiphoton microscopic imaging,MPM)initially,evaluating the diagnostic value in vitro.Methods:The multiphoton images of 100 fresh tissues confirmed pathologically colorectal normal tissues,hyperplastic polyps,adenomas and adenocarcinomas were collected and retrospectively analyzed to establish diagnostic standards with MPM.Results:Through the investigation,we found the colorectal normal,hyperplastic polyps,adenoma and adenocarcinoma were different in the crypt opening,glandular structure,epithelial cells and collagen fibers.Besides,the cellular areas by quantitive analysis were 32.14 ± 1.96 μm2、32.69 ± 2.34 μm2、55.40 ± 4.63 μm2、71.83 ± 5.30 μm2、85.90 ± 11.50 μm2,respectively.P values were both of statistically significance.Conclusion:The real-time multiphoton microscopic imaging can be used to classify and grade the colorectal diseases safely and effectively with high resolution.It is potential to become the new generation of optical technique via integrating with the endoscopes in the future.关键词
多光子显微成像技术/结直肠疾病/诊断/光学活检Key words
Multiphoton microscopic imaging/colorectal diseases/diagnosis/optical biopsy分类
医药卫生引用本文复制引用
何可心,范志宁,赵黎黎,黄晓阳,丁颖,化宏金,刘莉,王翔,王敏,张勇..多光子显微成像技术用于诊断结直肠疾病可行性的初步研究[J].南京医科大学学报（自然科学版）,2018,38(2):201-205,238,6.基金项目
国家自然科学基金(81700577,81302107) （81700577,81302107）
江苏省科技临床专项(BL2012031) （BL2012031）
江苏省“333”工程基金(BRA2015472) （BRA2015472）
江苏省青年医学人才项目(QNRC2016569) （QNRC2016569）
