农业环境科学学报2018，Vol.37Issue(3)：495-504,10.DOI:10.11654/jaes.2017-1107
稻作条件下不同施肥模式对盐碱化土壤细菌和古菌群落结构的影响
Effect of varying fertilization patterns on bacteria and archaea communities in saline-alkali soil under rice cul-tivation
摘要
Abstract
This study investigated the characteristics of soil bacterial and archaea community shifts subjected to different fertilization pat-terns in a saline- alkali soil under rice cultivation in Northwest China. There were five treatments: CK(without fertilizer), single flue gas desulfurization gypsum by-products(FGDB)31 250 kg·hm-2application(T1);flue gas desulfurization gypsum by-products 31 250 kg·hm-2, and different organic fertilizer treatments(T2,T3,and T4,respectively).Five cores(2.5 cm in diameter)were randomly collected from the plow layer(2~20 cm)of each plot in the experimental field and mixed together.The changes to the bacterial and archaea communities,and soil nutrients were investigated using Illumina pyrosequencing and conventional chemical analysis, respectively. When compared with the control, the other four treatments decreased the diversity index of soil archaeal community. However, the T2, T3, and T4 treatments in-creased the biodiversity of the soil bacterial community.Across all samples,Proteobacteria and Bacteroidetes were the most dominant bacte-rial phyla,and represented 25.7%~31.2% and 22.9%~28.1% of all taxon tags,respectively.T2,T3,and T4 treatments increased the rela-tive abundances of Proteobacteria by 14.0%~36.4%,but T1 decreased the relative abundance of Proteobacteria by 9.2% compared with CK. However,T1,T2,and T3 treatments increased the relative abundance of Euryarchaeota by about 41.6%~115.2% compared with CK.These results showed that soil bacterial and archaeal communities were significantly altered by the different fertilization patterns.The use of FGDB and organic fertilizer changed the soil physicochemical properties.T4 treatment increased soil organic carbon(6.74%), available nitrogen (37.2%), available phosphorus(47.83%), and NO-3- N(96.26%). There were significant correlations between soil microorganisms and physicochemical characteristics.The RDA results indicated that the NO-3-N came from the organic fertilizer applications and was the most important factor determining bacterial and archaeal community composition.The total phosphorus,pH,available phosphorus,and elec-trical conductivity also had important effects on the microorganism communities. Treatment 4 was the best fertilizer pattern. These results may increase our understanding of the microorganisms in a saline-alkali soil,and will improve the rational utilization of fertilizer and agri-cultural sustainable development.关键词
稻作/施肥模式/细菌和古菌群落/盐碱化土壤/高通量测序Key words
rice cultivation/fertilization pattern/bacterial and archaeal communities/saline-alkali soil/Illumina pyrosequencing分类
农业科技引用本文复制引用
李明,马飞,肖国举..稻作条件下不同施肥模式对盐碱化土壤细菌和古菌群落结构的影响[J].农业环境科学学报,2018,37(3):495-504,10.基金项目
宁夏自然科学基金项目(NZ17025)Project supported:The Natural Science Foundation of Ningxia(NZ17025) （NZ17025）
