农业环境科学学报2018，Vol.37Issue(3)：530-537,8.DOI:10.11654/jaes.2017-1091
凤眼莲根际耐Cd、Zn细菌的分离鉴定及对Cd、Zn去除效果研究
Isolation and identification of Cd- and Zn-resistant bacteria in the rhizosphere of Eichhornia crassipes and their effects on Cd and Zn removal
摘要
Abstract
Three Cd- and Zn-resistant bacteria strains(R12, R15, and R16)were isolated from the rhizosphere of Eichhornia crassipes. Heavy metal tolerance tests indicated that the strains could tolerate up to 10 mg·L-1and 30 mg·L-1of Cd2+and Zn2+,respectively,and 16S rRNA sequence analyses identified isolates R12 and R15 as Pseudomonas and isolate R16 as Stenotrophomonas.The results on the effect of strains on the removal of heavy metals during 36 h showed that the amount of Cd and Zn removed by R12 and R16 increased with time, whereas that removed by R15 first decreased and then increased,and the amount of Cd and Zn removed by all the strains reached a maxi-mum after 36 h.Among the three strains,R16 was the most capable of removing Cd and Zn,with maximum removal amounts of 0.17 mg·kg-1 and 2.71 mg·kg-1Cd and Zn,respectively,and maximum removal rates of 41% and 16%.Furthermore,the co-culture of E.crassipes and the rhizosphere isolates on Cd- and Zn-enriched substrate indicated that R12 and R16 mainly affect the Cd enrichment of E.crassipes by inhibiting the entry of Cd ions into the roots,whereas R15 affected Cd enrichment by regulating both the entry of Cd ions into the roots and the migration of Cd ions inside plants.The three strains all affected Zn enrichment by regulating the migration of Zn ions inside the plants.关键词
凤眼莲/耐重金属细菌/镉/锌/生物富集Key words
Eichhornia crassipes/heavy metal resistant bacteria/cadmium/zinc/biological enrichment分类
资源环境引用本文复制引用
郭山,桂恒俊,陈翩,赵建伟,周文兵,万小琼,朱端卫..凤眼莲根际耐Cd、Zn细菌的分离鉴定及对Cd、Zn去除效果研究[J].农业环境科学学报,2018,37(3):530-537,8.基金项目
国家自然科学基金项目(41201510) （41201510）
中央高校基本科研业务费专项资金(2011QC093) （2011QC093）
教育部博士学科点专项科研基金项目(2011014-6120037)Project supported:The National Natural Science Foundation of China(41201510) （2011014-6120037）
Fundamental Research Funds for the Central Universities(2011QC093) （2011QC093）
Specialized Research Fund for the Doctoral Program of Higher Education of China(20110146120037) （20110146120037）
