农药学学报2018，Vol.20Issue(1)：25-32,8.DOI:10.16801/j.issn.1008-7303.2018.0004
不同杀菌剂对黄瓜靶斑病菌的毒力作用特性比较
Comparison of toxicity properties of different types of fungicides against Corynespora cassiicola on cucumber
摘要
Abstract
In order to discover highly effective fungicides againstCorynespora cassiicola on cucumber, the inhibitory activities of 19 fungicides against mycelial growth, spore germination and germ tube elongation ofC. cassiicola were measured. The effects of the 4 selected effective fungicides on mycelial and conidial morphology were also evaluated. The results showed that fluazinam, isopyrazam, fludioxonil and tetramycin exhibited strong inhibitory activities against mycelial growth, spore germination and germ tube elongation at each growth stage ofC. cassiicola. The average EC50 value ofthe four fungicides for inhibiting mycelial growth were 0.5163, 1.538, 1.605 and 0.6482 mg/L, respectively. The average EC50 value of inhibiting the spore germination were 0.1644, 1.359, 1.127 and 0.0025 mg/L, respectively. The average EC50 value of inhibiting germ tube elongation were 0.1896, 0.1447, 0.0604 and 0.0015 mg/L, respectively. Fungicides with different modes of action, such as boscalid, chlorothalonil and trifloxystrobin, showed high efficacy against spore germination and tube elongation ofC. cassiicola. The average EC50 value of three fungicides to inhibit the spore germination were 1.564, 0.3730 and 0.0213 mg/L, respectively. The average EC50 value of inhibiting the germ tube elongation were 0.6295, 0.2334 and 0.4050 mg/L, respectively. But the inhibitory activities against mycelial growth were poor, the average EC50 value were greater than 16 mg/L. After the treatment of fluazinam, isopyrazam, fludioxonil or tetramycin, the morphology of mycelial and conidia was studied. The microscopical observation showed that the mycelial septa spacing decreased, the edge of diaphragm appeared as intumescent and two branches of the main mycelia frequently occurred. In addition, mycelia became distorted and adhesive, the new branches of mycelium were inhibited or conidial growth was metamorphotic. Given that fluazinam, isopyrazam, fludioxonil and tetramycin showed excellent inhibitory virulence againstC. cassiicola, these fungicides are potential candidates for the control ofC. cassiicola in the field.关键词
黄瓜靶斑病/杀菌剂/氟啶胺/吡唑萘菌胺/咯菌腈/四霉素/毒力/菌丝Key words
Corynespora cassiicola/fungicides/fluazinam/isopyrazam/fludioxonil/tetramycin/toxicity/mycelium分类
农业科技引用本文复制引用
禾丽菲,李晓旭,朱佳美,慕卫,刘峰..不同杀菌剂对黄瓜靶斑病菌的毒力作用特性比较[J].农药学学报,2018,20(1):25-32,8.基金项目
国家重点研发计划(2016YFD0200500) （2016YFD0200500）
山东省重大科技创新工程 (2017CXGC0207). （2017CXGC0207）
