农药学学报2018，Vol.20Issue(1)：41-48,8.DOI:10.16801/j.issn.1008-7303.2018.0008
稻曲病菌离体培养体系的构建与优化
Construction and optimization of a culture system for growth and bioassay of Ustilaginoidea virens in vitro
摘要
Abstract
Rice false smut is an important fungal disease at the rice heading stage, which causes severe loss to yield production and rice quality. Due to insufficient growth performance ofUstilaginoidea virens in vitro, screening and observation of effective fungicides in bioassay have been restricted. In this work, selection, combination and optimization of various carbon, nitrogen sources and coagulants have been carried out, an optimized culture system forU. virens growth was developed. The growth rate ofU. virens in 5 representative types of commercial media was only from 0.7 to 2.3 mm/d, while the significant growth promotion effect onU. virens was observed in 77％ of 18 types of compound media. Especially, maximum growth rate of 3.4 mm/d was observed in the peptone sucrose gellan gum (PSGG) medium. Interestingly, growth promotion effect of this medium was also observed on different strains of U. virens collected from rice grown in geographically diverse regions. Moreover, supplementation of 0.02％ MgSO4 into the media solidified using gellan gum could enhance the solidification degree of the culture system, but did not affect the growth ofU. virens. This indicated that peptone and sucrose were ideal nitrogen and carbon sources, respectively, in the PSGG medium, and gellan gum was a favorable coagulant. Our results would improve the growth rate ofU. virens in vitro, and advance the understanding of underlying mechanism involved in the growth ofU. virens, and also serve as an basis for the efficiency improvement of the bioassay system forU. virens.关键词
稻曲病菌/菌丝生长速率/培养基构建/蛋白胨/蔗糖/结冷胶/镁离子Key words
Ustilaginoidea virens/mycelium growth rate/construction of culture media/peptone/sucrose/gellan gum/magnesium ion分类
农业科技引用本文复制引用
刘晓玉,潘九月,MATSUMOTO Haruna,范小艳,林婷,钱圆,朱国念,王蒙岑..稻曲病菌离体培养体系的构建与优化[J].农药学学报,2018,20(1):41-48,8.基金项目
浙江省重大科技专项重点农业项目(2015C02019) （2015C02019）
国家重点研发计划 (2017YFD0202104) （2017YFD0202104）
浙江省自然科学基金项目(LQ16C140001) （LQ16C140001）
国家自然科学基金项目 (31501684) （31501684）
中央高校基本科研业务费专项资金. （）
