农药学学报2018，Vol.20Issue(1)：58-66,9.DOI:10.16801/j.issn.1008-7303.2018.0010
QuEChERS-超高效液相色谱-串联质谱法测定香菇中56种农药残留
Determination of 56 pesticides inLentinus edodes using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A simple and fast multi-residue method was established to simultaneously determine 56 pesticide residues, such as carbendazim, acetamiprid, and fipronil, inLentinus edodes using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Samples were extracted with acidic acetonitrile and then cleaned up with a mixture of sorbents (PSA and C18). Compounds were ionized using the electrospray ionization (ESI+) and operated in positive mode. Results showed that good linearity was achieved with a determination coefficient of 0.99. Recoveries ranged from 71％ to 121％ with relative standard deviation (RSD) of 0.6％ to 9.2％, which were goodenough for the routine analysis of the pesticide residues. The effect of solvents and filter membranes on the detection ofL. edodes were also investigated. When the solvent contains a high proportion of water phase, pyridaben and other compounds have a significant loss when filtered through the PTFE or nylon filter. The results of matrix effect study showed that 40 pesticides, such as methomyl, exhibited weak matrix effect. And 16 pesticides, such as methamidophos, showed moderate medium matrix effect. The method can be used as routine analysis method in multi-residual pesticide monitoring ofL. edodes.关键词
QuEChERS/超高效液相色谱-串联质谱/香菇/多残留/基质效应Key words
Lentinus edodes/QuEChERS/UPLC-MS/MS/multi-residue/matrix effect分类
农业科技引用本文复制引用
吴延灿,戚传勇,操海群,苏漪玲,商鲁宁,檀根甲,蒋冰心..QuEChERS-超高效液相色谱-串联质谱法测定香菇中56种农药残留[J].农药学学报,2018,20(1):58-66,9.基金项目
安徽省自然科学基金(1508085MC50). （1508085MC50）
