农药学学报2018，Vol.20Issue(1)：124-128,5.DOI:10.16801/j.issn.1008-7303.2018.0011
QuEChERS-液相色谱-串联质谱法检测糙米中氟唑环菌胺的残留
Determination of sedaxane in brown rice by QuEChERS-liquid chromatography-tandem mass spectrometry
摘要
Abstract
An analytical method for the determination of sedaxane residues in brown rice was established by QuEChERS and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with a mixture of acetonitrile and water (80/20,V/V), purified by dispersion solid phase extraction with PSA as sorbent, and quantified using LC-MS/MS by external standard method. The results showed that good linearity was obtained in the range from 0.001 to 0.1 mg/L, with R2 > 0.996. At the fortified levels of 0.004, 0.01 and 0.1 mg/kg, the recoveries of sedaxane in brown rice samples were 83％-95％ with the relative standard deviations (RSD) of 3.9％-12％. The limit of quantitation (LOQ) was 0.004 mg/kg in brown rice samples, which was significantly lower than themaximum residue limit (MRL) of 0.01 mg/kg set by the Codex Alimentarius Commission. The proposed method is simple, accurate and sensitive. Hence, it could be used for the detection of sedaxane residues in brown rice. A market survey of 40 brown rice samples from 6 provinces was carried out, and the residual amount of sedaxane was less than 0.004 mg/kg in all samples. The dietary risk assessment showed that the use of sedaxane in rice was currently at a low risk in China.关键词
QuEChERS/液相色谱-串联质谱/糙米/氟唑环菌胺/残留Key words
QuEChERS/liquid chromatography-tandem mass spectrometry (LC-MS/MS)/brown rice/sedaxane/residue分类
农业科技引用本文复制引用
姜桦韬,尤祥伟,张广雨,隋程程,王秀国,李义强..QuEChERS-液相色谱-串联质谱法检测糙米中氟唑环菌胺的残留[J].农药学学报,2018,20(1):124-128,5.基金项目
国家重点研发计划"化学肥料和农药减施增效综合技术研发"重点专项(2016YFD0200200-5). （2016YFD0200200-5）
