农药学学报2018，Vol.20Issue(1)：129-134,6.DOI:10.16801/j.issn.1008-7303.2018.0005
QuEChERS-高效液相色谱-串联质谱法测定主要谷物和油料作物中氯虫苯甲酰胺的残留
QuEChERS-determination of chlorantraniliprole residues in primary grain and oilseed crops by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A rapid, simple and efficient analytical method for the determination of chlorantraniliprole in different primary grain and oilseed crops including brown rice, wheat, maize and soybean was developed and validated based on QuEChERS and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Chlorantraniliprole residues were extracted from primary grain andoilseed crops samples using acetonitrile-water with the addition of sodium chloride. After centrifugation, the upper solvent was cleaned-up byN-propylethyl-diamine (PSA) and graphitizing of carbon black (GCB), and determined by HPLC-MS/MS under multiple reaction monitoring mode (MRM). Sample matrix-matched calibrations were used to determine the residue contents by external standard. The calibration curves showed a good linearity in the concentration range of 0.005-0.5 mg/L for chlorantraniliprole in different sample matrix. The determination coefficients (R2) of the calibration curves were all above 0.99 and the limit of detection (LOD) was 0.001 mg/L. At fortification levels of 0.02, 0.05, 0.1 and 0.5 mg/kg in different primary grain and oilseed crop samples, recoveries ranged from 89％ to 114％ with relative standard deviation (RSD) of 1.3％ to 11％ (n = 5). The limit of quantification (LOQ) of chlorantraniliprole in brown rice, wheat, maize and soybean were all 0.02 mg/kg. This new method is applicable to detect the residues of chlorantraniliprole in different primary grain and oilseed crops.关键词
QuEChERS/高效液相色谱-串联质谱/氯虫苯甲酰胺/谷物/油料作物/残留Key words
QuEChERS/high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)/chlorantraniliprole/grain/oilseed crops/residue分类
农业科技引用本文复制引用
马婧玮,马欢,安莉,吴家锴,汪红,祁玉峰,陈寅,吴绪金..QuEChERS-高效液相色谱-串联质谱法测定主要谷物和油料作物中氯虫苯甲酰胺的残留[J].农药学学报,2018,20(1):129-134,6.基金项目
粮油作物产品未知危害因子识别与已知危害因子安全性评估项目(GJFP2015007) （GJFP2015007）
河南省农业科学院基础科研项目 (2016). （2016）
