中国海洋大学学报（自然科学版）2018，Vol.48Issue(3)：73-79,7.DOI:10.16441/j.cnki.hdxb.20170008
喹烯酮与鸡鸭血浆蛋白结合率的检测
Determination of Plasma Protein Binding Rates of Quinocetone in Chicken and Duck
摘要
Abstract
Objective:To establish an assay method for the determination of quinocetone in poultry plasma,and to determine the plasma protein binding rate of quinocetone.Method:The equilibrium dialysis method was used to determine plasma protein binding rate of quinocetone invitro;the biological samples was extracted with acetonitrile,the analytes was separated by high performance liquid chromatography and the detection methodology is verified.Results:A method for the determination of quinocetone in the plasma was established and the linear range was 0.3～10μg/mL,correlation coefficient r＞0.999 5;average recovery rates around 86.5％～92.2％ in spiked concentration of 0.3、1 and 2μg/mL,and the relative standard deviation were below 5 ％.The average plasma protein binding rate of quinocetone in chicken and duck were 40.53％±1.09％ and 46.88％±1.25％.There was no significant difference between the plasma protein binding rate of quinocetone in the concentration range of 0.5～8 μg/mL.The result shows that the probability of drug interaction is low and the application of quinocetone in poultry has high security.关键词
喹烯酮/血浆蛋白结合率/平衡透析/HPLCKey words
quinocetone/the plasma protein binding rate/equilibrium dialysis/HPLC分类
化学化工引用本文复制引用
张瑞丽,郝智慧,曲少奇,王远红,姜廷福,吕志华..喹烯酮与鸡鸭血浆蛋白结合率的检测[J].中国海洋大学学报（自然科学版）,2018,48(3):73-79,7.基金项目
国家重点研发计划项目(2016YFD0501309) （2016YFD0501309）
国家自然科学基金项目(31402256) （31402256）
青岛农业大学高层次人才研究项目(631206)资助 Supported by the National Key Research and Development Plan (2016YFD0501309) （631206）
National Natural Science Foundation of China (31402256) （31402256）
High-Level Talent Research Foundation of Qingdao Agricultural University (631206) （631206）
