器官移植2018，Vol.9Issue(2)：116-121,6.DOI:10.3969/j.issn.1674-7445.2018.02.005
氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响
Effect of oxygen glucose deprivation-reperfusion in astrocytes overexpressing endothelin-1 on the proliferation of neural stem/progenitor cells
摘要
Abstract
Objective To investigate the effect of oxygen glucose deprivation-reperfusion (OGD-R) in astrocytes overexpressing endothelin (ET)-1 on the proliferation of neural stem/progenitor cells (NSPCs). Methods OGD-R models of negative control astrocytes (C6-Mock) and astrocytes over-expressing ET-1 (C6-ET-1) were constructed. Transwell co-culture system of astrocytes and NSPCs was established. Morphologic observation and identification of the astrocytes and primary NSPCs were performed. The cells were divided into four groups: C6-Mock+NSPCs, OGD-R+C6-Mock+NSPCs, C6-ET-1+NSPCs and OGD-R+C6-ET-1+NSPCs groups and co-cultured for 0, 24, 48 and 72 h respectively. The diameter of neurosphere was measured in each group. Results In the C6-Mock and C6-ET-1 cells, type Ⅰ astrocytes in fibrous morphology were observed. Glial fibrillary acidic protein (GFAP) was expressed in the cytoplasm of these two types of cells. Primary NSPCs were positive for nestin staining. After co-culture for 48 and 72 h, the neurosphere diameter in the OGD-R+C6-Mock+NSPCs group was significantly greater than that in the C6-Mock+NSPCs group. The neurosphere diameter in the OGD-R+C6-ET-1+NSPCs group was considerably greater than that in the C6-ET-1+NSPCs group. The neurosphere diameter in the OGD-R+C6-ET-1+NSPCs group was significantly greater compared with that in the OGD-R+C6-Mock+NSPCs group (all P<0.05). Conclusions OGD-R astrocytes can promote the proliferation of NSPCs. ET-1 over-expression further accelerates the proliferation of NSPCs.关键词
星型胶质细胞/神经干/祖细胞(NSPCs)/内皮素(ET)-1/氧糖剥夺-再灌注(OGD-R)/巢蛋白/增殖/Transwell共培养/神经球直径Key words
Astrocyte/Neural stem/progenitor cells (NSPCs)/Endothelin (ET)-1/Oxygen glucose deprivation-reperfusion (OGD-R)/Nestin/Proliferation/Transwell co-culture/Neurosphere diameter分类
医药卫生引用本文复制引用
程骁,杨家杰,钟金淑子..氧糖剥夺-再灌注星型胶质细胞内皮素-1过表达对神经干/祖细胞增殖的影响[J].器官移植,2018,9(2):116-121,6.基金项目
国家自然科学基金(81303115、81774042、81771353) （81303115、81774042、81771353）
广东省自然科学基金(S2013040016915) （S2013040016915）
广州市科技计划(201604020003) （201604020003）
中国博士后项目(BBK42913K09、201003345、BBH429151701) （BBK42913K09、201003345、BBH429151701）
香江学者计划 （）
