热带作物学报2017，Vol.38Issue(10)：1796-1803,8.DOI:10.3969/j.issn.1000-2561.2017.10.005
‘砂糖橘’夏梢生长对幼果和离区细胞壁降解酶活性及基因表达的影响
Effects of Summer Shoot Growth on Activities and Gene Expression of the Cell Wall Degradation Enzymes in ‘Shatangju’ Mandarin Fruitlet and Abscission Zone
摘要
Abstract
The objective of this work was to investigate the effects of shoot growth on the activities,gene expression of the cell wall degradation enzymes and abscission of fruitlet and abscission zone (AZ) in Citrus.10-year old trees of ‘Shatangju’ mandarin (Citrus reticulata Blanco) were used as the material.The results showed that summer shoot obviously increased fruitlet abscission.At the 15th day,the accumulative abscission rate (AAR) in shoot-retained treatment was 79.5％,which was 91.7％ higher than that in shoot-removed treatment.Moreover,the activities of cellulase (Cx) and polygalacturonase (PG) in fruitlet and AZ were dramatically increased during the summer shoot growth,and the activities of those two enzymes were positively correlated with the relative abscission rate (RAR).However,the activities of pectin methylesterase (PME) and β-galactosidase (β-Gal) were not influenced by summer shoot growth.qRT-PCR analysis showed that the expression levels of CsPG,Csβ-Gal and CsCx were significantly upregulated during the summer shoot growth,with the expression peaks were appeared at the 6th day,but a little effect was found in the expression of CsPME.These results suggested that during fruitlet abscission induced by summer shoot growth in ‘Shatangju’ mandarin,Cx and PG were the key cell wall degradation enzymes which regulated fruitet abscission.关键词
砂糖橘/夏梢/细胞壁降解酶/基因表达/脱落Key words
Shatangju/summer shoot/cell wall degradation enzymes/gene expression/abscission分类
农业科技引用本文复制引用
黄永敬,陈杰忠,曾继吾,吴文,张瑞敏,朱从一..‘砂糖橘’夏梢生长对幼果和离区细胞壁降解酶活性及基因表达的影响[J].热带作物学报,2017,38(10):1796-1803,8.基金项目
国家现代农业(柑橘)产业技术体系建设专项(No.CARS-27) （柑橘）
广东省自然科学基金-博士启动项目(No.2015A030310432) （No.2015A030310432）
广东省科技厅产学研合作项目(No.2014B090908016、2015B090906025、2015B090906026). （No.2014B090908016、2015B090906025、2015B090906026）
