热带作物学报2017，Vol.38Issue(10)：1901-1906,6.DOI:10.3969/j.issn.1000-2561.2017.10.021
胡椒实时荧光定量PCR内参基因的筛选
The Identification of Internal Control Genes for Real-time PCR Normalization in Black Pepper
摘要
Abstract
In order to identify the suitable internal control gene for the gene expression analysis in black pepper,the expression of 9 housekeeping genes,including Polyubiquitin 1,Polyubiquitin 2,Polyubiquitin 3,GAPDH 1,GAPDH 2,Histone 3-1,Histone 3-2,Cyclophilin and Actin were assessed by using real time quantitative PCR in 8 different tissues.On the basis of Ct value comparison,the stability of housekeeping gene expression was analyzed by using geNorm,NormFinder and BestKeeper.The results of comprehensive ranking by three methods indicated that the most stable three reference genes were Histone 3-1,Polyubiquitin 1 and GAPDH 2.The value of pairwise variation Vn/n+1 suggested that Histone 3-1 and Polyubiquitin 1 had to be used together for normalization,which would make the expression of target genes more reliable in black pepper.关键词
胡椒/实时荧光定量PCR/内参基因Key words
Black pepper/real-time PCR/internal control gene分类
农业科技引用本文复制引用
胡丽松,范睿,伍宝朵,吴刚,谭乐和,郝朝运..胡椒实时荧光定量PCR内参基因的筛选[J].热带作物学报,2017,38(10):1901-1906,6.基金项目
中国热带农业科学院基本科研业务费专项资金(No.1630142017015) （No.1630142017015）
国家自然科学基金(No.31601626). （No.31601626）
