热带作物学报2017，Vol.38Issue(10)：1932-1939,8.DOI:10.3969/j.issn.1000-2561.2017.10.026
水黄连抑菌物质提取工艺及其抑菌效果的研究
Optimizing Extraction Conditions of Antifungal Substance from Thalictrum smithii B.by Response Surface Methodology and Its Inhibitory Effect
摘要
Abstract
Penicillium italicum was used as the tested strain.According to the single-factor experiments,a four-variable,three-level Box-Benhnken Design (BBD) matrix was applied to optimize the extraction conditions of antifungal substance from Thalictrum smithii B.by a response surface methodology.The control efficacy of the TSB extract was tested in vivo against blue mold in citrus fruit.The statistical analysis showed that the factors influencing the extracting antifungal substance were in the order:ultrasound extraction time＞ethanol concentration＞ liquid-to-solid ratio＞temperature.The optimum extraction conditions were as following:ethanol concentration of 65％,extraction time of 82 min,liquid-to-solid ratio of 23 mL/g and temperature of 75 ℃.Under the above extraction conditions,the average value of inhibitory zone diameter was (52.33±0.41)mm,well matched with the predicted value of 52.20 mm.The TSB extract had significant efficacy in controlling blue mold in citrus fruit and the EC50 value was 59.53 mg/mL in vivo test.关键词
水黄连/超声波提取/响应面法/意大利青霉菌/抑菌效果Key words
Thalictrum smithii B./ultrasonic extraction/RSM/Penicillium italicum/inhibitory effect分类
医药卫生引用本文复制引用
陈楚英,万春鹏,付永琦,甘武,彭旋,陈金印..水黄连抑菌物质提取工艺及其抑菌效果的研究[J].热带作物学报,2017,38(10):1932-1939,8.基金项目
江西省现代农业柑橘产业技术体系(2016) （2016）
江西省青年科学基金项目(No.20171BAB214031) （No.20171BAB214031）
江西省教育厅科技落地计划项目(111) （111）
江西省“赣鄱英才555工程”(2012). （2012）
