摘要
Abstract
Two HPLC-MS/MS methods for the analysis of fenaminstrobin residue in fruits and vegetables were developed and validated.The samples were extracted with acetonitrile and cleared up by the methods of QuEChERS extraction and solid phase extraction (SPE) on amino column.The chromatographic separation was carried out by Thermo Scientific Hypersll GOLD column.Fenaminstrobin was detected by electro-spray ionization (ESI+),and selected the reaction monitoring (SRM) mode.Quantification was performed by peak area external standard methods using matrix-matched calibration curves.The results showed that the matrix effects were-9.5％～-42.4％ and-14.9％～-54.1％,respectively,using the two different purification methods.Good linear relationship (R2＞0.998) was achieved in the range of 1～400 μg/L,and the limit of detection (LOD) of the two methods was 0.06 μg/kg and 0.02 μg/kg,respectively.At 2,20 and 200 μg/kg spiked levels,the recoveries was 83.8％～100.3％,82.4％～102.4％,and the relative standard deviations (RSD) was 2.6％～7.1％,4.1％～10.2％,respectively.Both of the methods were sensitive and reliable,and were suitable for the determination of residues in fruits and vegetables.关键词
烯肟菌胺/高效液相色谱-串联质谱/残留/蔬菜水果/基质效应Key words
Fenaminstrobin/high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)/residue/fruits and vegetables/matrix effect分类
化学化工
