热带作物学报2017，Vol.38Issue(11)：2082-2088,7.DOI:10.3969/j.issn.1000-2561.2017.11.015
木薯eIF4E7基因RNAi载体构建及沉默效果分析
Construction of RNAi Vector Targeting Cassava eIF4E7 and Identifcation of Its Interfering Efficiency
摘要
Abstract
Cassava (Manihot esculenta Grantz) production in large areas of Africa is severely affected by cassava brown streak disease (CBSD) which is caused by two virus species,cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV).At present,there is no effective strategy for preventing CBSD.To complete the translation and replicaiton of its genome,virus need to employ the translation and replication system of host.During virus infection plant's eukaryotic initiation factor 4E (eIF4E) has been implicated a necessary factor for a variety of RNA viruses.Cassava eIF4E7 is one member of Cassava eIF4E family.At present study we develop a interfering construct p1300-Ca4E7 which targetting Cassava eIF4E7 gene and demonstrate the construct can interfere the expression of eIF4E7 gene efficiently by employing the N.Banthemiana transient expression system and the semi-quantitative RT-PCR as well.The result pave a way for further investigating the function of Cassava eIF4E7 during the infecton of CBSV and UCBSV.It also provide theoretical basis for the new strategy of anti-virus by targeting host genes with RNAi technology.关键词
木薯/eIF4E7/干扰载体/本生烟瞬时表达/半定量RT-PCR/沉默效果Key words
Cassava (Manihot esculenta Crantz)/eIF4E7/interference vecter/N.Banthemiana Transient Expression System/semi-quantitative RT-PCR/interference Efficiency分类
农业科技引用本文复制引用
张秀春,武亚丹,张春微,王健华,余乃通,刘志昕..木薯eIF4E7基因RNAi载体构建及沉默效果分析[J].热带作物学报,2017,38(11):2082-2088,7.基金项目
国家自然科学基金项目资助(No.31461143016,No.31570145). （No.31461143016,No.31570145）
