热带作物学报2017，Vol.38Issue(11)：2089-2099,11.DOI:10.3969/j.issn.1000-2561.2017.11.016
福州野生蕉隐花色素和光敏色素基因家族的克隆及其表达分析
Cloning and Expression Analysis of Cryptochrome and Phytochrome Family Genes in the Wild Banana (Musa spp.) from Fuzhou City
摘要
Abstract
The RT-PCR combined with RACE method was used to obtain Cryptochrome and Phytochrome gene family in the wild banana of Fuzhou,named MuCry1,MuCry2a,MuCry2b,MuPhyB and MuPhyC1.respectively.The cDNA sequences of MuCrys and MuPhys were 2 330,2 859,2 725,3 272,3 912 bp,encoding 698,666,669,1 089 and 1 003 amino acids.Bioinformatics analysis showed that All MuCrys and MuPhys belong to the instability and hydrophilous proteins,and had transmembrane structures,and had no signal peptide,subcellularlocation predictions of them locating were in the nucleus.Phylogenetic anglicizing of Cry and Phy in plants indicated that not only Cry1 and Cry2,but also PhyB and PhyC belonged to the different branches,but they all had farthest genetic relationship with dicotyledons;MuCryl have a close genetic relationship with MaCry1 (Musa acuminata),EgCry1 (Elaeis guineensis),and PdCry1 (Phoenix dactylifera),MuCry2a and MuCry2b belongs to the same branch with a close genetic relationship with MuCry2,and TaCry2 (Triticum aestivum),and OsCry2 (Oryza sativaJaponica),MuPhyB have a close genetic relationship with MuPhyB (Musa acuminata),MuPhyC1 have a close genetic relationship with MuPhyC (Musa acuminata) and OmPhyC (Orchidantha maxillarioides).qRT-PCR results revealed that three members of Cryptochrome gene family and MuPhyC1 had different responses to different light quality,and blue light could promote the transcription of MuCrys mRNA,and red light could promote the transcription of MuPhyC1.The expression of MuCry1 was inhibited in dark,red,green and yellow light;The effect of light on the transcription level of MuCry2a was not obvious,while dark,warm white and green light could suppress the expression level of MuCry2a.The transcriptional level of MuCry2b was positively regulated by blue and red light,and was negatively controled by green light;MuPhyC1 had the highest expression in red light,The expression level of white red,green,yellow and dark light treatment was not significant,indicating that MuPhyC1 positively responded to red light stimulation.关键词
野生蕉/隐花色素/光敏色素/光质处理/表达分析Key words
Wild banana (Musa spp)/Crypthchrome/Phytochrome/light quality treatment/gene expression分类
农业科技引用本文复制引用
刘转霞,余运康,陈裕坤,冯新,刘炜婳,张梓浩,程春振,林玉玲,赖钟雄..福州野生蕉隐花色素和光敏色素基因家族的克隆及其表达分析[J].热带作物学报,2017,38(11):2089-2099,11.基金项目
国家香蕉产业技术体系专项资金(No.CARS-32-11) （No.CARS-32-11）
福建省重大科技专项(No.2015NZ2002-1) （No.2015NZ2002-1）
