热带作物学报2017，Vol.38Issue(11)：2100-2105,6.DOI:10.3969/j.issn.1000-2561.2017.11.017
巴西橡胶树HbCYP450基因克隆与表达分析
Cloning and Expression Analysis of HbP450 Gene in Hevea brasiliensis Muell.Arg.
摘要
Abstract
Cytochrome P450 monooxygenases catalyze a wide varieties of monooxygenation reactions in primary and secondary metabolism in plants.In this study,a CYP450 gene was cloned from rubber tree leaves.It encodes 516 amino acids which has one conserved domain at position 3-511 and a special conserved sequence of heme binding sites.HbCYP450 has identity of 74％,68％,68％,and 65％ with Ricinus communis L.,Populus euphratica,Populus trichocarpa and Glycine max,respectively.HbCYP450 expressed in leaf,flower and latex,but no expressed in bark,which expression of gene induced by drought,wounding and powdery mildew disease.Under the treatment of IAA,ABA,SA,JA,ET and H2O2,the expression patterns of HbCYP450 were significantly upregulated,then downregulated dramatically.These results suggest that HbCYP450 is one of the members of P450 family,and the expression are induced by powdery mildew and stress induction.关键词
巴西橡胶树/基因克隆/表达分析/CYP450Key words
Hevea brasiliensis/gene cloning/expression analysis/CYP450分类
农业科技引用本文复制引用
李晓娜,肖厚贞,万三连,张冬,张宇..巴西橡胶树HbCYP450基因克隆与表达分析[J].热带作物学报,2017,38(11):2100-2105,6.基金项目
国家自然科学基金(No.31660204,31660187) （No.31660204,31660187）
中国热带农业科学院基本科研业务费专项资金(No.1630072016005). （No.1630072016005）
