热带作物学报2017，Vol.38Issue(11)：2119-2123,5.DOI:10.3969/j.issn.1000-2561.2017.11.020
‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导
Explant Process Optimization and Induction of Adventitious Bud of Pitaya ‘Jindu No.1’
摘要
Abstract
This study used red-flesh dragon fruit variety-“Jindu No.1” stem as the material,to research the best way of explant selection,explant disinfection treatment,adventitious bud induction in the pitaya tissue culture experiment.The results showed that pitaya explant had better choose healthy and robust nearly mature stem;and the most suitable disinfection treatment for pitaya stem segments was keeping thorn and followed by rinsing in 75％ alcohol for 30 s,then rinsed 0.1％ mercuric chloride disinfection for 10 min,and finally rinsed with sterile water 5-8 times.The best culture medium for adventitious bud induction of pitaya was MS+4.0 mg/L 6-BA+0.1 mg/L NAA,and induction rate was 80％.关键词
火龙果/外植体/消毒方式/不定芽诱导Key words
pitaya/explant/disinfection/adventitious bud induction分类
农业科技引用本文复制引用
洪青梅,胡文斌,李洪立,汪琴琴,何云,濮文辉,李琼..‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导[J].热带作物学报,2017,38(11):2119-2123,5.基金项目
科技部、财政部国家科技资源共享服务平台-国家农作物种质资源共享服务平台(No.NICGR2017-67) （No.NICGR2017-67）
农业部物种品种资源保护(热带作物)项目(No.151721301354052012) （热带作物）
海南省重点研发项目(No.ZDYF2017058) （No.ZDYF2017058）
中国热带农业科学院基本科研业务费专项资金(No.1630032017062) （No.1630032017062）
2017年所非营利项目(No.pzsfyl-201704) （No.pzsfyl-201704）
中国热带农业科学院基本科研业务费专项资金(No.1630032016001). （No.1630032016001）
