热带作物学报2017，Vol.38Issue(11)：2151-2159,9.DOI:10.3969/j.issn.1000-2561.2017.11.025
1株拮抗细菌的分离鉴定及其对香蕉枯萎病等病原菌的抑制活性测定
Screening, Identification of an Antagonistic Bacteria and Determinning a Method to Inhibit 12 Pathogenic Fungus,Such as Fusarium oxysporum f.sp.cubense
摘要
Abstract
In order to increase the effective component of the Biocontrol Medicament,four antagonistic bacteria were isolated from the food of soybean paste in this article.The method of tablet confrontation was used to determine the bacterial strain of the bacterium,and to obtain a resistant bacteria with stable inhibitory activity.Preliminary identification of strain Y-4 was Bacillus amyloliquefaciens through the observation of morphology,and the analysis of physiological and biochemical characteristics,and 16S rDNA sequence.Measuring the different state and operation in the inhibition activity of the strain.The results showed:The strain of Y-4 had strong inhibiting activity in 12 Pathogenic fungus,such as Fusarium oxysporum f.sp.cubense,The bacteriostatic rate was 53.33％～86.67％.Among them,the bacteriostatic rate of Fusarium oxysporum f.sp.cubense was 63.33％,the Fermentation liquid has certain bacteriostas was activity.Performed the Inhibition effect to different concentrations of crude protein,and the inhibition effect to Fusarium oxysporum f.sp.cubense was the best in 2 000 mg/mL.The antibacterial rate of the twelve pathogenic fungi was up to 70％ with Ethanol extract.The strain of Y-4 was isolated from the food had stable bacteriostatic activity,to develop new research fields for the Biocontrol Medicament of Fusarium oxysporum f.sp.cubense.关键词
甲基营养型芽胞杆菌/拮抗/生防制剂/广谱Key words
Bacillus methylotrophicus/antagonism/biocontrol medicament/broad spectrum分类
农业科技引用本文复制引用
张妙宜,陈志杰,陈宇丰,冯仁军,高祝芬,张锡炎..1株拮抗细菌的分离鉴定及其对香蕉枯萎病等病原菌的抑制活性测定[J].热带作物学报,2017,38(11):2151-2159,9.基金项目
现代农业产业技术体系建设专项资金项目(No.CARS-32). （No.CARS-32）
