水产学报2018，Vol.42Issue(4)：476-484,9.DOI:10.11964/jfc.20170510848
香鱼白细胞介素17C基因克隆及鳗利斯顿氏菌侵染后的时空表达
Cloning of IL-17 C gene from Plecoglossus altivelis and the expression pattern analysis after Listonella anguillarum infection
摘要
Abstract
To understand the sequence features and phylogeny of interleukin 17(IL-17)in sweet fish(Plecoglos-sus altivelis),and its correlation to Listonella anguillarum infection,the cDNA sequence of IL-17C was cloned from P.altivelis by RACE-PCR,and the expression changes of different tissues were detected after L.anguillar-um infection by real-time quantitative PCR(qRT-PCR).The full length of P.altivelis IL-17C cDNA was 1 087 bp including a 534 bp ORF and seven 3′UTR instability motifs (ATTTA), and it encoded 177 amino acids with eight conservation cysteine (Cys) and a 19 amino acids N-terminal signal peptide. Phylogenetic tree analysis showed that the same IL-17 subtype homologue from different species gathered together,and the P.altivelis IL-17C was clustered in one group with other vertebrate IL-17C. Multiple sequence alignment and phylogenetic analysis showed that P.altivelis IL-17C has higher similarity to Oncorhynchus mykiss IL-17C1(40.72%)and O.mykiss IL-17C2(34.04%).In healthy P.altivelis,IL-17C mRNA expression was detected in heart,liver,spleen,head kidney, gill,brain,muscle and intestine,high expression was shown in gill and head kidney.After L.anguillarum infec-tion,the expression of IL-17C increased 9.17 folds in liver at 8 hpi(hours post infection)and 6.69 folds in spleen at 4 hpi compared to the control group.IL-17C gene is closely related to the pathogenic bacteria infection of P.altivelis,and it may play an important role in inflammatory immune response.关键词
香鱼/白细胞介素17C基因/基因克隆/鳗利斯顿氏菌/时空表达Key words
Plecoglossus altivilis/interleukin 17C(IL-17C)/gene cloning/Listonella anguillarum/expression pat-tern分类
生物科学引用本文复制引用
李明云,苗亮,郭晓飞,聂力,侯红红,陈炯..香鱼白细胞介素17C基因克隆及鳗利斯顿氏菌侵染后的时空表达[J].水产学报,2018,42(4):476-484,9.基金项目
国家自然科学基金(41406154) （41406154）
宁波市自然科学基金(2015A610271) （2015A610271）
浙江省教育厅(理)科研计划(Y201430860) （理）
浙江省"水产"重中之重学科开放基金(xkzsc1514)National Natural Science Foundation of China (41406154) （xkzsc1514）
Natural Science Foundation of Ningbo (2015A610271) （2015A610271）
Research Project (Science) of Department of Education of Zhejiang Province (Y201430860) （Science）
Open Fund of Priority Subjects (Aquaculture) of Zhejiang Province (xkzsc1514) （Aquaculture）
